Methodological evaluation of gingival crevicular fluid volume and neutrophil elastase levels: sequential sampling, length of sampling time and two different sampling methods

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dc.contributor.authorKayar, Nezahat Arzu
dc.contributor.authorOduncuoğlu, Bahar Füsun
dc.contributor.authorHaliloğlu, Seyfullah
dc.contributor.authorSerpek, Behiç
dc.contributor.authorAtaoğlu, Tamer
dc.contributor.authorAlptekin, Nilgün Özlem
dc.date.accessioned2020-03-26T20:15:12Z
dc.date.available2020-03-26T20:15:12Z
dc.date.issued2019
dc.departmentSelçuk Üniversitesi, Veteriner Fakültesi, Temel Bilimler Bölümüen_US
dc.description.abstractObjectives: The mechanisms underlying the formation and composition of gingival crevicular fluid (GCF) and its flow into and from periodontal pockets are not understood very well. The aim of this study was to evaluate the length of sampling time and sequential sampling of GCF neutrophil elastase (NE) enzyme levels by using intracrevicular and orifice methods. Material and methods: Twenty adults (mean age of 41.8 years, ranged 31-60 years, 18 males and 2 females) with chronic periodontitis were enrolled and all completed the 3-d study. GCF was collected by both intracrevicular and intrasulcular methods, 720 samples of GCF were collected. In first, second and third day, the length of sampling time in seconds (s) and order were '5- 10-30-s'; '10- 30- 5-s' and '30- 5- 10-s,' respectively. GCF elastase levels were determined by hydrolysis of neutrophil specific substrate N-methoxysuccinyl-Ala-Ala-Pro-Val-p-nitroanilide. Results: NE activity (mu U) and NE activity/volume (mu U/mu l) were significantly different for order of sampling (p < .05), but not for the length of sampling time (p>.05). Conclusions: Within the limits of this study, the choice of sampling technique in GCF-profile studies seems to be a critical decision as it has the potential to affect the GCF volume and NE activity.en_US
dc.identifier.citationKayar, N. A., Oduncuoğlu, B. F., Haliloğlu, S., Serpek, B., Ataoğlu, T., Alptekin, N. Ö. (2020). Methodological Evaluation Of Gingival Crevicular Fluid Volume And Neutrophil Elastase Levels: Sequential Sampling, Length Of Sampling Time And Two Different Sampling Methods. Acta Odontologica Scandinavica, 78,(4), 290-296.
dc.identifier.doi10.1080/00016357.2019.1703141en_US
dc.identifier.issn0001-6357en_US
dc.identifier.issn1502-3850en_US
dc.identifier.pmid31852321en_US
dc.identifier.urihttps://dx.doi.org/10.1080/00016357.2019.1703141
dc.identifier.urihttps://hdl.handle.net/20.500.12395/37998
dc.identifier.wosWOS:000503362900001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorHaliloglu, Seyfullah
dc.institutionauthorSerpek, Behic
dc.language.isoenen_US
dc.publisherTAYLOR & FRANCIS LTDen_US
dc.relation.ispartofACTA ODONTOLOGICA SCANDINAVICAen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectGingival crevicular fluiden_US
dc.subjectsampling methodsen_US
dc.subjectsequential samplingen_US
dc.subjectsamplingen_US
dc.titleMethodological evaluation of gingival crevicular fluid volume and neutrophil elastase levels: sequential sampling, length of sampling time and two different sampling methodsen_US
dc.typeArticleen_US

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