Modulatory effects of rutin on the expression of cytochrome P450s and antioxidant enzymes in human hepatoma cells

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dc.contributor.authorKarakurt, Serdar
dc.date.accessioned2020-03-26T19:25:09Z
dc.date.available2020-03-26T19:25:09Z
dc.date.issued2016
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractExpression of a drug and xenobiotic metabolizing enzymes, cytochrome P450s (CYPs), and antioxidant enzymes can"be modulated by various factors. The flavonoid rutin was investigated for its anti-carcinogen and protective effects as well as modulatory action on CYPs and phase II enzymes in human hepatocellular carcinoma cel ls. Rutin inhibited proliferation of HEPG2 cells in a dose-dependent manner with the IC50 value of 52.7 mu mol L-1 and invasion of HEPG2 cells (21.6 %, p = 0.0018) and colony formation of those invaded cells (57.4 %, p < 0.0001). Rutin treatment also significantly increased early/late-stage apoptosis in HEPG2 cells (28.9 %, p < 0.001). Treatment by rutin significantly inhibited protein expressions of cytochrome P450-dependent CYP3A4 (75.3 %, p < 0.0001), elevated CYP1A1 enzymes (1.7-fold, p = 0.0084) and increased protein expressions of antioxidant and phase II reaction catalyzing enzymes, NQO1 (2.42-fold, p < 0.0001) and GSTP1 (2.03-fold, p < 0.0001). Besides, rutin treatment significantly inhibited mRNA expression of CYP3A4 (73.2 %, p=0.0014). Also, CYP1A1, NQO1 and GSTP1 mRNA expressions were significantly increased 2.77-fold (p = 0.029), 4.85 fold (p = 0.0051) and 9.84-fold (p < 0.0001), respectively.en_US
dc.description.sponsorshipResearch Foundation of Selcuk UniversitySelcuk University [14401126]en_US
dc.description.sponsorshipThis work is supported by The Research Foundation of Selcuk University (project number: 14401126).en_US
dc.identifier.doi10.1515/acph-2016-0046en_US
dc.identifier.endpage502en_US
dc.identifier.issn1330-0075en_US
dc.identifier.issn1846-9558en_US
dc.identifier.issue4en_US
dc.identifier.pmid27749250en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage491en_US
dc.identifier.urihttps://dx.doi.org/10.1515/acph-2016-0046
dc.identifier.urihttps://hdl.handle.net/20.500.12395/33795
dc.identifier.volume66en_US
dc.identifier.wosWOS:000386863500004en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherHRVATSKO FARMACEUTSKO DRUSTOV (HFD)-CROATION PHARMACEUTICAL SOCen_US
dc.relation.ispartofACTA PHARMACEUTICAen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectrutinen_US
dc.subjectcytochrome P450sen_US
dc.subjectphase II enzymesen_US
dc.subjectcell proliferationen_US
dc.subjectHEPG2en_US
dc.titleModulatory effects of rutin on the expression of cytochrome P450s and antioxidant enzymes in human hepatoma cellsen_US
dc.typeArticleen_US

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