Inhibitory action of Epilobium hirsutum extract and its constituent ellagic acid on drug-metabolizing enzymes

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dc.contributor.authorÇelik, Gurbet
dc.contributor.authorSemiz, Aslı
dc.contributor.authorKarakurt, Serdar
dc.contributor.authorÖzkan, Ayşe Mine Gençler
dc.contributor.authorArslan, Şevki
dc.contributor.authorAdalı, Orhan
dc.contributor.authorŞen, Alaattin
dc.date.accessioned2020-03-26T19:24:43Z
dc.date.available2020-03-26T19:24:43Z
dc.date.issued2016
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractEpilobium hirsutum (EH) is a medicinal plant for treating various diseases. Despite its wide usage, there is no available information about its potential influences on drug metabolism. The present study was undertaken to determine the in vivo effects of EH on hepatic CYP2B, CYP2C, CYP2D, and CYP3A enzymes that are primarily involved in drug metabolism. Male Wistar rats were injected intraperitoneally with EH water extract (EHWE) and ellagic acid (EA) at a daily dose of 37.5 and 20 mg/kg, respectively, for 9 days and hepatic drug-metabolizing enzymes were assessed at activity, protein and mRNA levels. Erythromycin N-demethylase activity was inhibited by 53 and 21 % in EHWE- and EA-treated rats, respectively. Benzphetamine N-demethylase and 7-benzyloxy-resorufin-O-debenzylase activities were decreased by 53 and 43 %, and 57 and 57 % in EHWE-and EA-treated rats, respectively. Moreover, protein levels of CYP2B1, CYP2C6, CYP2D2, and CYP3A1 also decreased by 55, 15, 33, and 82 % as a result of EHWE treatment of rats, respectively. Similarly, CYP2B1, CYP2C6, CYP2D2, and CYP3A1 protein levels decreased by 62, 63, 49, and 37 % with EA treatment, respectively. qRT-PCR analyses also showed that mRNA levels of these enzymes were significantly inhibited with bothEHWE and EA treatments. In conclusion, inhibition of drug clearances leading to drug toxicity because of the lowered activity and expression of drug-metabolizing enzymes might be observed in the people who used EH as complementary herbal remedy that might be contributed by its EA content.en_US
dc.description.sponsorshipScientific and Technological Research Council of TurkeyTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [109R012]; Pamukkale UniversityPamukkale University [2010FBE081]en_US
dc.description.sponsorshipThis work was supported by the Scientific and Technological Research Council of Turkey [109R012] and Pamukkale University [2010FBE081].en_US
dc.identifier.doi10.1007/s13318-014-0238-1en_US
dc.identifier.endpage116en_US
dc.identifier.issn0378-7966en_US
dc.identifier.issn2107-0180en_US
dc.identifier.issue2en_US
dc.identifier.pmid25425117en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage109en_US
dc.identifier.urihttps://dx.doi.org/10.1007/s13318-014-0238-1
dc.identifier.urihttps://hdl.handle.net/20.500.12395/33712
dc.identifier.volume41en_US
dc.identifier.wosWOS:000376251300002en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSPRINGER FRANCEen_US
dc.relation.ispartofEUROPEAN JOURNAL OF DRUG METABOLISM AND PHARMACOKINETICSen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectEpilobium hirsutumen_US
dc.subjectEllagic aciden_US
dc.subjectDrug-metabolizing enzymesen_US
dc.subjectInhibition, drug-herb interactionen_US
dc.subjectCYP2B1en_US
dc.subjectCYP2C6en_US
dc.subjectCYP2D2en_US
dc.subjectCYP3A1en_US
dc.titleInhibitory action of Epilobium hirsutum extract and its constituent ellagic acid on drug-metabolizing enzymesen_US
dc.typeArticleen_US

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