Enhanced catalysis and enantioselective resolution of racemic naproxen methyl ester by lipase encapsulated within iron oxide nanoparticles coated with calix[8]arene valeric acid complexes

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dc.contributor.authorSayın, Serkan
dc.contributor.authorAköz, Enise
dc.contributor.authorYılmaz, Mustafa
dc.date.accessioned2020-03-26T18:50:29Z
dc.date.available2020-03-26T18:50:29Z
dc.date.issued2014
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractIn this study, two types of nanoparticles have been used as additives for the encapsulation of Candida rugosa lipase via the sol-gel method. In one case, the nanoparticles were covalently linked with a new synthesized calix[8]arene octa valeric acid derivative (C[8]-C-4-COOH) to produce new calix[8]arene-adorned magnetite nanoparticles (NP-C[8]-C-4-COOH), and then NP-C[8]-C-4-COOH was used as an additive in the sol-gel encapsulation process. In the other case, iron oxide nanoparticles were directly added into the sol-gel encapsulation process in order to interact electrostatically with both C[8]-C-4-COOH and Candida rugosa lipase. The catalytic activities and enantioselectivities of two novel encapsulated lipases (Enc-NP-C[8]-C-4-COOH and Enc-C[8]-C-4-COOH@Fe3O4) in the hydrolysis reaction of racemic naproxen methyl ester were evaluated. The results showed that the activity and enantioselectivity of the lipase were improved when the lipase was encapsulated in the presence of calixarene-based additives. Indeed, the encapsulated lipases have an excellent rate of enantioselectivity, with E = 371 and 265, respectively, as compared to the free enzyme (E = 137). The lipases encapsulated with C[8]-C-4-COOH and iron oxide nanoparticles (Enc-C[8]-C-4-COOH@Fe3O4) retained more than 86% of their initial activities after 5 repeated uses and 92% with NP-C[8]-C-4-COOH.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [111T027]; Scientific and Technological Research Council of Turkey (TUBITAK, CMST COST Action)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [CM1005]; Research Foundation of Selcuk University (BAP)Selcuk Universityen_US
dc.description.sponsorshipThis work was funded by the Scientific and Technological Research Council of Turkey (TUBITAK grant no.111T027 and CMST COST Action CM1005), and the Research Foundation of Selcuk University (BAP).en_US
dc.identifier.doi10.1039/c4ob01048een_US
dc.identifier.endpage6642en_US
dc.identifier.issn1477-0520en_US
dc.identifier.issn1477-0539en_US
dc.identifier.issue34en_US
dc.identifier.pmid25012138en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage6634en_US
dc.identifier.urihttps://dx.doi.org/10.1039/c4ob01048e
dc.identifier.urihttps://hdl.handle.net/20.500.12395/30820
dc.identifier.volume12en_US
dc.identifier.wosWOS:000341021400013en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherROYAL SOC CHEMISTRYen_US
dc.relation.ispartofORGANIC & BIOMOLECULAR CHEMISTRYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.titleEnhanced catalysis and enantioselective resolution of racemic naproxen methyl ester by lipase encapsulated within iron oxide nanoparticles coated with calix[8]arene valeric acid complexesen_US
dc.typeArticleen_US

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