Effects of different concentrations of BHT on microscopic and oxidative parameters of Mahabadi goat semen following the freeze-thaw process

Basit Öğe Kaydı
dc.contributor.authorNaijian, Hamid Reza
dc.contributor.authorKohram, Hamid
dc.contributor.authorShahneh, Ahmad Zare
dc.contributor.authorSharafi, Mohsen
dc.contributor.authorBucak, Mustafa Numan
dc.date.accessioned2020-03-26T18:41:42Z
dc.date.available2020-03-26T18:41:42Z
dc.date.issued2013
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractOxidative damage to sperm is one of the main causes for decline in motility and fertility of frozen thawed sperm. Thus, it is crucial to use cryoprotectant agents in extender in order to prevent lethal intracellular ice crystal formation. The present study aims to evaluate the effects of different concentrations of the antioxidant butylated hyroxytoluene (BHT) on sperm parameters post-thaw. Semen was diluted into five equal aliquots of extender containing different concentrations of BHT (0, 0.5, 1, 2 and 4 mM), aspirated into 0.25 mL straws, and equilibrated at 5 degrees C for 2 h. After equilibration, straws were frozen in liquid nitrogen vapor and plunged into liquid nitrogen for storage. Sperm parameters, including motility and progressive motility, viability, membrane integrity, acrosome integrity and capacitation status, were assessed. Malondialdehiyde (MDA) and glutathione peroxidase (GSH-PX) activity were also evaluated after freezing-thawing. Results of this experiment show that addition of 1 mM of BHT to the extender for freezing of goat semen can improve motility, progressive motility and viability (P < 0.05) and reduce the MDA level (P < 0.01). HOST (hypo-osmotic swelling test), acrosome integrity, capacitation status and GSH-PX were not affected by the concentrations of BHT (P > 0.05). Therefore, we conclude that the optimum concentration of BHT for cryopreservation of goat semen is 1 mM. (C) 2013 Elsevier Inc. All rights reserved.en_US
dc.identifier.doi10.1016/j.cryobiol.2012.12.010en_US
dc.identifier.endpage155en_US
dc.identifier.issn0011-2240en_US
dc.identifier.issn1090-2392en_US
dc.identifier.issue2en_US
dc.identifier.pmid23313785en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage151en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.cryobiol.2012.12.010
dc.identifier.urihttps://hdl.handle.net/20.500.12395/29439
dc.identifier.volume66en_US
dc.identifier.wosWOS:000316518100013en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherACADEMIC PRESS INC ELSEVIER SCIENCEen_US
dc.relation.ispartofCRYOBIOLOGYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectGoat spermen_US
dc.subjectOxidative damageen_US
dc.subjectCryopreservationen_US
dc.subjectButylated hydroxytolueneen_US
dc.subjectProgressive motilityen_US
dc.titleEffects of different concentrations of BHT on microscopic and oxidative parameters of Mahabadi goat semen following the freeze-thaw processen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu