Titanium dioxide nanoparticles induce cytotoxicity and reduce mitotic index in human amniotic fluid-derived cells

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dc.contributor.authorAcar, Mustafa Said
dc.contributor.authorBulut, Zeynep Betül
dc.contributor.authorAteş, A.
dc.contributor.authorNami, Babak
dc.contributor.authorKoçak, Nadir
dc.contributor.authorYıldız, B.
dc.date.accessioned2020-03-26T19:07:44Z
dc.date.available2020-03-26T19:07:44Z
dc.date.issued2015
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractTitanium dioxide (TiO2) nanoparticles (NPs) are commonly used materials present in many consumables for which most people are exposed to. The biological hazards of the NPs on human health have been demonstrated previously. In this study, we aimed to assess the cytotoxicity potency of TiO2 NPs on the primary human amniotic fluid cells. The cells derived from amniotic fluid were treated with different dosages of TiO2 NPs for some periods. Cell adhesion status was assessed using a light microscopic observation. Cell proliferation and cell death rates were determined using trypan blue staining and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Also, mitotic index was determined using fluorescence in situ hybridization with chromosome 8 centromer-specific DNA probe. Disrupted cell adhesion, decreased proliferation, and increased mortality rates were detected in the cells that were treated with TiO2 NPs depending on the dosage (p < 0.001). Also, reduced mitotic index was determined in the cells depending on the time and TiO2 dosage when compared with the controls (p < 0.0001). These results showed that TiO2 NPs have high cytotoxicity for amniotic fluid-derived cells. Therefore, different products containing TiO2 NPs should be used with care, especially for pregnant women.en_US
dc.identifier.doi10.1177/0960327114530742en_US
dc.identifier.endpage82en_US
dc.identifier.issn0960-3271en_US
dc.identifier.issn1477-0903en_US
dc.identifier.issue1en_US
dc.identifier.pmid24717318en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage74en_US
dc.identifier.urihttps://dx.doi.org/10.1177/0960327114530742
dc.identifier.urihttps://hdl.handle.net/20.500.12395/32701
dc.identifier.volume34en_US
dc.identifier.wosWOS:000346643700008en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSAGE PUBLICATIONS LTDen_US
dc.relation.ispartofHUMAN & EXPERIMENTAL TOXICOLOGYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectTitanium dioxideen_US
dc.subjectamniotic fluid cellsen_US
dc.subjectmitotic indexen_US
dc.subjectcytotoxicityen_US
dc.subjectFISHen_US
dc.subjectMTT assayen_US
dc.titleTitanium dioxide nanoparticles induce cytotoxicity and reduce mitotic index in human amniotic fluid-derived cellsen_US
dc.typeArticleen_US

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