Effects of different setting of diode laser on the mRNA expression of growth factors and type I collagen of human gingival fibroblasts

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dc.contributor.authorHakki, Sema S.
dc.contributor.authorBozkurt, S. Buket
dc.date.accessioned2020-03-26T18:25:13Z
dc.date.available2020-03-26T18:25:13Z
dc.date.issued2012
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractThe aim of this study was to analyze the influence of non-surgical applications of diode laser (940 nm) on the cell proliferation and mRNA expressions of type I collagen and growth factors in human gingival fibroblasts (GF). Gingival fibroblasts were isolated from human gingival connective tissue of systemically healthy individuals. Cells were treated with different laser parameters as follows; (1) Infected pocket setting (power: 2 W, pulse interval: 1 ms, pulse length: 1 ms, 20 s/cm(2)); (2) Perio-pocket setting (power: 1.5 W, pulse interval: 20 ms, pulse length: 20 ms, 20 s/cm(2)); and (3) Biostimulation setting (power: 0.3 W in continuous wave, 20 s/cm(2)). Proliferation of GF was evaluated after different laser applications using a real-time cell analyzer. Total RNA was isolated on day 2 and cDNA synthesis was performed. Type I collagen, insulin-like growth factor (IGF), vascular endothelial growth factor (VEGF) and transforming growth factor-beta (TGF-beta) mRNA expressions were determined with quantitative RT-PCR. In a proliferation experiment, no significant differences were observed in the different laser applications when compared to the control group. Statistically significant increases in IGF, VEGF, and TGF-beta mRNA expressions were noted in the laser groups when compared to the untreated control group (p < 0.05). A significant increase in collagen type I mRNA expression was noted in only biostimulation set-up of diode laser (p < 0.05). The results of this study demonstrate that non-surgical laser applications modulate behavior of gingival fibroblasts inducing growth factors mRNA expressions and these applications can be used to improve periodontal wound healing.en_US
dc.identifier.doi10.1007/s10103-010-0879-5en_US
dc.identifier.endpage331en_US
dc.identifier.issn0268-8921en_US
dc.identifier.issn1435-604Xen_US
dc.identifier.issue2en_US
dc.identifier.pmid21246387en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage325en_US
dc.identifier.urihttps://dx.doi.org/10.1007/s10103-010-0879-5
dc.identifier.urihttps://hdl.handle.net/20.500.12395/27974
dc.identifier.volume27en_US
dc.identifier.wosWOS:000300588500009en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSPRINGER LONDON LTDen_US
dc.relation.ispartofLASERS IN MEDICAL SCIENCEen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectDiode laseren_US
dc.subjectLow-level laser therapyen_US
dc.subjectGingival fibroblastsen_US
dc.subjectGrowth factorsen_US
dc.subjectType I collagenen_US
dc.subjectProliferationen_US
dc.titleEffects of different setting of diode laser on the mRNA expression of growth factors and type I collagen of human gingival fibroblastsen_US
dc.typeArticleen_US

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