Porphyromonas gingivalis Lipopolysaccharide Induces a Pro-inflammatory Human Gingival Fibroblast Phenotype

Basit Öğe Kaydı
dc.contributor.authorBozkurt, S. Buket
dc.contributor.authorHakki, Sema S.
dc.contributor.authorHakki, Erdogan E.
dc.contributor.authorDurak, Yusuf
dc.contributor.authorKantarci, Alpdogan
dc.date.accessioned2020-03-26T19:42:20Z
dc.date.available2020-03-26T19:42:20Z
dc.date.issued2017
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractHuman gingival fibroblasts (HGFs) are the major constituents of the gingival tissues responsible for the synthesis and degradation of the connective tissue while actively participating in immune reactions and inflammation. The aim of this study was to test the impact of lipopolysaccharide (LPS) from Porphyromonas gingivalis (P. gingivalis) on human gingival fibroblasts. Human gingival fibroblasts were treated with different P. gingivalis LPS concentrations. Cell survival rate was evaluated with 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) after 24 h. Cell proliferation was determined by counting cells on days 3 and 12. Expression of matrix metalloproteinases (MMPs), tissue inhibitors of MMPs (TIMPs), and pro-inflammatory cytokine transcripts in HGFs was determined by quantitative PCR (Q-PCR) analysis on days 3 and 8. P. gingivalis LPS decreased cell proliferation on day 3 (p < 0.05) compared to the control group without significantly impacting the cell survival (p > 0.05).The experiments showed that P. gingivalis LPS dose-dependently and differentially modulated the expression of MMP-1, 2, and 3 and TIMP-1 and 2 on days 3 and 8. TIMP-1 expression was significantly induced in P. gingivalis LPS-treated cells while TIMP-2 was increased in response to 10 and 30 ng/ml of LPS on day 3. P. gingivalis LPS induced up-regulation of MMP-1/TIMP-1 ratio on day 3 and increased MMP-2/TIMP-2 ratio on day 8 dose-dependently. Expression of interleukin (IL)-6 and IL-8 was stimulated at higher concentrations (1000 and 3000 ng/ml) of LPS. These findings demonstrate that P. gingivalis LPS suppresses cell proliferation and leads to increased pro-inflammatory changes in HGFs, suggesting that P. gingivalis LPS-induced modification of phenotypic and inflammatory characteristics in HGF could potentially be a pathogenic mechanism underlying the tissue destruction.en_US
dc.description.sponsorshipResearch Fund, Selcuk University [BAP/08201022]en_US
dc.description.sponsorshipThis study was performed at the Selcuk University Faculty of Dentistry, Research Center and was supported by the Research Fund, Selcuk University (BAP/08201022).en_US
dc.identifier.doi10.1007/s10753-016-0463-7en_US
dc.identifier.endpage153en_US
dc.identifier.issn0360-3997en_US
dc.identifier.issn1573-2576en_US
dc.identifier.issue1en_US
dc.identifier.pmid27812843en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage144en_US
dc.identifier.urihttps://dx.doi.org/10.1007/s10753-016-0463-7
dc.identifier.urihttps://hdl.handle.net/20.500.12395/35388
dc.identifier.volume40en_US
dc.identifier.wosWOS:000393655500015en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSPRINGER/PLENUM PUBLISHERSen_US
dc.relation.ispartofINFLAMMATIONen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectPorphyromonas gingivalisen_US
dc.subjectlipopolysaccharideen_US
dc.subjectfibroblasten_US
dc.subjectcytokineen_US
dc.titlePorphyromonas gingivalis Lipopolysaccharide Induces a Pro-inflammatory Human Gingival Fibroblast Phenotypeen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu