Screening and genotyping of group B streptococcus in pregnant and nonpregnant women in Turkey

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dc.contributor.authorAlp, Feyza
dc.contributor.authorFindik, Duygu
dc.contributor.authorDagi, Hatice Turk
dc.contributor.authorArslan, Ugur
dc.contributor.authorPekin, Aybike Tazegul
dc.contributor.authorYilmaz, Setenay Arzu
dc.date.accessioned2020-03-26T19:26:11Z
dc.date.available2020-03-26T19:26:11Z
dc.date.issued2016
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractIntroduction: The purpose of this study was to investigate group B streptococcus (GBS) colonization, to compare the methods, to determine the relationship between GBS carriage and risk factors, and to genotype the GBS isolates. Methodology: Recto-vaginal swab specimens were obtained from 500 women, and a questionnaire was administered to each to assess their risk factors for GBS carriage. A culture, GBS antigen test, and polymerase chain reaction (PCR) were performed on all samples. Antibiotic susceptibility testing was performed, and the clonal relationship was determined by pulsed-field gel electrophoresis (PFGE) on all viable isolates. Results: Of the 500 women, sixty-eight (13.6%) women were GBS carriers, of whom 9.8% were pregnant and 16.5% not. There was a significant difference between GBS carriage and history of premature rupture of membrane (PROM). GBS was isolated from 65 (13%) samples. GBS was positive in 70 (14%) samples by antigen test and in 62 (12.4%) by PCR. Sixty-eight of the 70 positive antigen tests were confirmed by PCR or culture. Fifty-five isolates were resistant to tetracycline, 16 to erythromycin and clindamycin, and 13 to levofloxacin. Thirteen different pulsotypes and 17 sporadic strains were determined by PFGE. Conclusions: GBS carriage rate in non-pregnant women was higher than in pregnant women. The GBS antigen test was more sensitive than culture and PCR. GBS isolates did not originate from a single clone and contained sporadic strains. There was a significant difference between GBS carriage and history of PROM. Epidemiologic data obtained in this study will help future studies.en_US
dc.description.sponsorshipSelcuk University Scientific Research Projects Coordinator shipSelcuk Universityen_US
dc.description.sponsorshipThis study was supported by Selcuk University Scientific Research Projects Coordinator ship. A part of this study was presented as a poster in the 16th International Congress on Infectious Diseases (April 2014) in Cape Town, South Africa.en_US
dc.identifier.doi10.3855/jidc.6190en_US
dc.identifier.endpage226en_US
dc.identifier.issn1972-2680en_US
dc.identifier.issue3en_US
dc.identifier.pmid27031453en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage222en_US
dc.identifier.urihttps://dx.doi.org/10.3855/jidc.6190
dc.identifier.urihttps://hdl.handle.net/20.500.12395/33968
dc.identifier.volume10en_US
dc.identifier.wosWOS:000373446200004en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherJ INFECTION DEVELOPING COUNTRIESen_US
dc.relation.ispartofJOURNAL OF INFECTION IN DEVELOPING COUNTRIESen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectGroup B streptococcusen_US
dc.subjectcarriageen_US
dc.subjectcultureen_US
dc.subjectPCRen_US
dc.subjectGBS antigen testen_US
dc.subjectPFGEen_US
dc.titleScreening and genotyping of group B streptococcus in pregnant and nonpregnant women in Turkeyen_US
dc.typeArticleen_US

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