Investigation of chicken infectious anemia virus infection by PCR and ELISA in chicken flocks
dc.contributor.author | Hadimli, H. Hueseyin | |
dc.contributor.author | Erganis, Osman | |
dc.contributor.author | Gueler, Leyla | |
dc.contributor.author | Ucan, U. Sait | |
dc.date.accessioned | 2020-03-26T17:27:05Z | |
dc.date.available | 2020-03-26T17:27:05Z | |
dc.date.issued | 2008 | |
dc.department | Selçuk Üniversitesi | en_US |
dc.description.abstract | Presence of the chicken infectious anemia virus (CIAV) infection in chicken flocks in some provinces of Turkey was investigated by ELISA and PCR in this study. From 38 flocks ( 16 commercial layer, 10 commercial broilers, 4 breeder layers and 8 breeder broilers), 922 serum samples were tested for CIAV-specific antibodies using a commercially available competitive ELISA. CIAV antibodies were positive in 609 (66.0%) sera from 34 flocks (89.5%). A total of 95 thymus samples from 25 flocks, 57 samples from 15 commercial layers, and 38 samples from 10 commercial broiler flocks were tested by PCR. In 53 (55.8%) thymus samples from 20 flocks (80.0%) CIAV-specific DNA was detected. In commercial layers antibodies were detected in 15 (93.7%) of 16 flocks and viral DNA was detected in 13 (86.6%) of 15 flocks. In commercial broilers both CIAV antibodies and DNA were detected in 7 (70%) of 10 flocks tested. While seropositivity was detected in 12 (100%) of breeding broilers ( 8) and layer ( 4) flocks tested, no PCR was performed in these flocks. The study showed high prevalence of subclinical CIAV infection in the investigated chicken flocks. | en_US |
dc.identifier.endpage | 84 | en_US |
dc.identifier.issn | 1300-0128 | en_US |
dc.identifier.issue | 2 | en_US |
dc.identifier.scopusquality | Q3 | en_US |
dc.identifier.startpage | 79 | en_US |
dc.identifier.uri | https://hdl.handle.net/20.500.12395/22467 | |
dc.identifier.volume | 32 | en_US |
dc.identifier.wos | WOS:000254304500003 | en_US |
dc.identifier.wosquality | Q4 | en_US |
dc.indekslendigikaynak | Web of Science | en_US |
dc.indekslendigikaynak | Scopus | en_US |
dc.indekslendigikaynak | TR-Dizin | en_US |
dc.language.iso | en | en_US |
dc.publisher | SCIENTIFIC TECHNICAL RESEARCH COUNCIL TURKEY-TUBITAK | en_US |
dc.relation.ispartof | TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.selcuk | 20240510_oaig | en_US |
dc.subject | chicken infectious anemia virus | en_US |
dc.subject | ELISA | en_US |
dc.subject | PCR | en_US |
dc.subject | chicken | en_US |
dc.title | Investigation of chicken infectious anemia virus infection by PCR and ELISA in chicken flocks | en_US |
dc.type | Article | en_US |