Investigation of chicken infectious anemia virus infection by PCR and ELISA in chicken flocks

dc.contributor.authorHadimli, H. Hueseyin
dc.contributor.authorErganis, Osman
dc.contributor.authorGueler, Leyla
dc.contributor.authorUcan, U. Sait
dc.date.accessioned2020-03-26T17:27:05Z
dc.date.available2020-03-26T17:27:05Z
dc.date.issued2008
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractPresence of the chicken infectious anemia virus (CIAV) infection in chicken flocks in some provinces of Turkey was investigated by ELISA and PCR in this study. From 38 flocks ( 16 commercial layer, 10 commercial broilers, 4 breeder layers and 8 breeder broilers), 922 serum samples were tested for CIAV-specific antibodies using a commercially available competitive ELISA. CIAV antibodies were positive in 609 (66.0%) sera from 34 flocks (89.5%). A total of 95 thymus samples from 25 flocks, 57 samples from 15 commercial layers, and 38 samples from 10 commercial broiler flocks were tested by PCR. In 53 (55.8%) thymus samples from 20 flocks (80.0%) CIAV-specific DNA was detected. In commercial layers antibodies were detected in 15 (93.7%) of 16 flocks and viral DNA was detected in 13 (86.6%) of 15 flocks. In commercial broilers both CIAV antibodies and DNA were detected in 7 (70%) of 10 flocks tested. While seropositivity was detected in 12 (100%) of breeding broilers ( 8) and layer ( 4) flocks tested, no PCR was performed in these flocks. The study showed high prevalence of subclinical CIAV infection in the investigated chicken flocks.en_US
dc.identifier.endpage84en_US
dc.identifier.issn1300-0128en_US
dc.identifier.issue2en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage79en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12395/22467
dc.identifier.volume32en_US
dc.identifier.wosWOS:000254304500003en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.publisherSCIENTIFIC TECHNICAL RESEARCH COUNCIL TURKEY-TUBITAKen_US
dc.relation.ispartofTURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCESen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectchicken infectious anemia virusen_US
dc.subjectELISAen_US
dc.subjectPCRen_US
dc.subjectchickenen_US
dc.titleInvestigation of chicken infectious anemia virus infection by PCR and ELISA in chicken flocksen_US
dc.typeArticleen_US

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