Osteogenic Differentiation of MC3T3-E1 Cells on Different Titanium Surfaces

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dc.contributor.authorHakkı, S. Sema
dc.contributor.authorBozkurt, S. Buket
dc.contributor.authorHakkı, Erdoğan E.
dc.contributor.authorKorkusuz, Petek
dc.contributor.authorPuralı, Nuhan
dc.contributor.authorKoç, Nursen
dc.contributor.authorTimuçin, Muharrem
dc.contributor.authorÖztürk, Adnan
dc.contributor.authorKorkusuz, Feza
dc.date.accessioned2020-03-26T18:30:59Z
dc.date.available2020-03-26T18:30:59Z
dc.date.issued2012
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractmRNA expressions related to osteogenic differentiation of MC3T3-E1 cells on electro-polished smooth (S), sandblasted small-grit (SSG) and sandblasted large-grit (SLG) surfaces of titanium alloys were investigated in vitro. Gene expression profiles of cells were evaluated using the RT2 Profiler PCR microarray on day 7. Mineralizing tissue-associated proteins, differentiation factors and extracellular matrix enzymes mRNA expressions were measured using Q-PCR. SLG surface upregulated 23 genes over twofolds and downregulated 3 genes when compared to the S surface. In comparison to the SSG surface, at least a twofold increase in 25 genes was observed in the SLG surface. BSP, OCN, OPN, COL I and ALP mRNA expressions increased in the SLG group when compared to the S and the SSG groups. BMP-2, BMP-6 and TGF-beta mRNA expressions increased in both the SSG and the SLG surfaces. MMP-2 and MMP-9 mRNA expressions increased as the surface roughness increased. This study demonstrated that surface roughness of titanium implants has a significant effect on cellular behavior and SLG surface apparently increased gene expressions related to osteogenesis when compared to the S and the SSG surfaces.en_US
dc.description.sponsorshipTurkish Scientific Research CouncilTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [TUBITAK-SBAG/3130]en_US
dc.description.sponsorshipThis study was performed at the Research Laboratory of Faculty of Dentistry, Selcuk University and was supported by Turkish Scientific Research Council (TUBITAK-SBAG/3130). The authors have no conflict of interests.en_US
dc.identifier.citationS. Hakkı, S., Bozkurt, S. B., Hakkı, E. E., Korkusuz, P., Puralı, N., Koç, N., Timuçin, M., Öztürk, A., Korkusuz, F., (2012). Osteogenic Differentiation of MC3T3-E1 Cells on Different Titanium Surfaces. Biomedical Materials, 7(4), 1-9. Doi:10.1088/1748-6041/7/4/045006
dc.identifier.doi10.1088/1748-6041/7/4/045006en_US
dc.identifier.endpage9
dc.identifier.issn1748-6041en_US
dc.identifier.issn1748-605Xen_US
dc.identifier.issue4en_US
dc.identifier.pmid22570189en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1
dc.identifier.urihttps://dx.doi.org/10.1088/1748-6041/7/4/045006
dc.identifier.urihttps://hdl.handle.net/20.500.12395/28281
dc.identifier.volume7en_US
dc.identifier.wosWOS:000306712900006en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorHakkı, S. Sema
dc.institutionauthorBozkurt, S. Buket
dc.institutionauthorHakkı, Erdoğan E.
dc.language.isoenen_US
dc.publisherIOP PUBLISHING LTDen_US
dc.relation.ispartofBiomedical Materialsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.titleOsteogenic Differentiation of MC3T3-E1 Cells on Different Titanium Surfacesen_US
dc.typeArticleen_US

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