Effects of cysteine and ergothioneine on post-thawed Merino ram sperm and biochemical parameters

Basit Öğe Kaydı
dc.contributor.authorCoyan, Kenan
dc.contributor.authorBaşpınar, Nuri
dc.contributor.authorBucak, Mustafa Numan
dc.contributor.authorAkalın, Pınar Peker
dc.date.accessioned2020-03-26T18:14:21Z
dc.date.available2020-03-26T18:14:21Z
dc.date.issued2011
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractThe aim of the current study was to evaluate the effects of cysteine and ergothioneine on the post-thawed sperm parameters, lipid peroxidation and antioxidant activities. Semen samples from 5 mature Merino rams were used in the study. Semen samples, which were diluted with a Tris-based extender containing L-Cysteine and L-(+)-Ergothioneine and no antioxidant (control), were cooled to 5 degrees C and frozen in 0.25 ml French straws. Frozen straws were then thawed individually at 37 degrees C for 20 s in a water bath for evaluation. Ergothioneine at doses of 2 and 4 mM increased percentages of subjective motility, VSL and VCL, compared to controls following the freeze-thawing (P < 0.001). Ergothioneine at three different doses led to higher rates of progressive motility and VAP, compared to control groups (P < 0.001). Cysteine and ergothioneine at three doses provided the higher rates of ALH, in comparison to no antioxidant group (P < 0.001). As regards CASA motility, supplementation with antioxidants did not provide any significant difference on the percentage of post-thaw sperm CASA motilities, in comparison to the control. In regards of sperm membrane integrity, only cysteine 1 mM provided a greater protective effect, compared to control (P < 0.001). Percentages of sperm with high mitochondrial activity were dramatically increased with cysteine at doses of 1 and 2 mM, compared to control (P < 0.05). No significant differences were observed in sperm acrosome integrities among groups. CAT activity was increased significantly only in cysteine1 mM compared to control group (P < 0.001). Cysteine at doses of 2 and 4 mM showed a tendency of increased activities of CAT when compared to control. But these increases were not statistically significant. Supplementation with antioxidants did not significantly affect activities of SOD and GPx. Findings of this study showed that ergothioneine supplementation in semen extenders, was of greater benefit to motility and motion characteristics of frozen-thawed ram sperm. Crown Copyright (C) 2011 Published by Elsevier Inc. All rights reserved.en_US
dc.description.sponsorshipSelcuk University, Directorate of Scientific Research Projects (BAP)Selcuk University; Scientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [108 O 522]en_US
dc.description.sponsorshipThis study was financed under the project supported by Selcuk University, Directorate of Scientific Research Projects (BAP) and by the Scientific and Technological Research Council of Turkey (TUBITAK) (Project No: 108 O 522).en_US
dc.identifier.doi10.1016/j.cryobiol.2011.04.001en_US
dc.identifier.endpage6en_US
dc.identifier.issn0011-2240en_US
dc.identifier.issn1090-2392en_US
dc.identifier.issue1en_US
dc.identifier.pmid21513707en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.cryobiol.2011.04.001
dc.identifier.urihttps://hdl.handle.net/20.500.12395/26397
dc.identifier.volume63en_US
dc.identifier.wosWOS:000292020800001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherACADEMIC PRESS INC ELSEVIER SCIENCEen_US
dc.relation.ispartofCRYOBIOLOGYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectCysteineen_US
dc.subjectErgothioneineen_US
dc.subjectFreezing-thawingen_US
dc.subjectRam spermen_US
dc.titleEffects of cysteine and ergothioneine on post-thawed Merino ram sperm and biochemical parametersen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu