Two distinct types of dying back axonal degeneration in vitro

Basit Öğe Kaydı
dc.contributor.authorOzturk, G.
dc.contributor.authorCengiz, N.
dc.contributor.authorErdogan, E.
dc.contributor.authorHim, A.
dc.contributor.authorOguz, E. K.
dc.contributor.authorYenidunya, E.
dc.contributor.authorAysit, N.
dc.date.accessioned2020-03-26T18:44:18Z
dc.date.available2020-03-26T18:44:18Z
dc.date.issued2013
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractG. ozturk, N. Cengiz, E. Erdoan, A. Him, E. K. Ouz, E. Yenidunya and N. Ayit (2013) Neuropathology and Applied Neurobiology39, 362376 Two distinct types of dying back axonal degeneration in vitro Aims: In many neurodegenerative diseases and following traumas, dying back degeneration is a common phenomenon that aggravates the pathology and may eventually lead to death of the affected neurone. We aimed to investigate the mechanism of dying back degeneration with an in vitro axonal injury model. Methods: We cultured adult mouse dorsal root ganglion neurones and with a precise laser beam, cut the axons they extended. Preparations were imaged continuously and images were analysed to describe and quantify ensuing events. Potential contributions of calpains and caspases to the degeneration were explored using specific inhibitors and immunohistochemistry. In vivo implications of the results were sought in nerve sections after sciatic nerve cut. Results: The proximal part of the transected axons went under basically two types of dying back degeneration, fragmentation and retraction. In fragmentation the cytoplasm became condensed and with concomitant axial collapse the axon disintegrated into small pieces. In retraction, the severed axon was pulled back to the soma in an organized manner. We demonstrated that fragmentation was associated with a high risk of cell death, while survival rate with retraction was as high as those of uninjured neurones. Regeneration of transected axon was more likely after retraction than following fragmentation. Activities of caspase-3 and calpains but not of caspase-6 were found linked with retraction and regeneration but not with the fragmentation. Conclusions: This study describes two quite distinct types of dying back degeneration that lead an injured neurone to quite different fates.en_US
dc.description.sponsorshipYuzuncu Yil University Directorate of Scientific Research ProjectsYuzuncu Yil University [TF073]en_US
dc.description.sponsorshipThis study was supported by Yuzuncu Yil University Directorate of Scientific Research Projects with the grant TF073.en_US
dc.identifier.doi10.1111/j.1365-2990.2012.01295.xen_US
dc.identifier.endpage376en_US
dc.identifier.issn0305-1846en_US
dc.identifier.issn1365-2990en_US
dc.identifier.issue4en_US
dc.identifier.pmid22845867en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage362en_US
dc.identifier.urihttps://dx.doi.org/10.1111/j.1365-2990.2012.01295.x
dc.identifier.urihttps://hdl.handle.net/20.500.12395/29974
dc.identifier.volume39en_US
dc.identifier.wosWOS:000318425900004en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWILEYen_US
dc.relation.ispartofNEUROPATHOLOGY AND APPLIED NEUROBIOLOGYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectaxotomyen_US
dc.subjectcaspaseen_US
dc.subjectdegenerationen_US
dc.subjectdying backen_US
dc.subjectlaseren_US
dc.subjectneurone deathen_US
dc.titleTwo distinct types of dying back axonal degeneration in vitroen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu