Rating of putative housekeeping genes for quantitative gene expression analysis in cyclic and early pregnant equine endometrium

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dc.contributor.authorKayis, Seyit A.
dc.contributor.authorAtli, Mehmet O.
dc.contributor.authorKurar, Ercan
dc.contributor.authorBozkaya, Faruk
dc.contributor.authorSemacan, Ahmet
dc.contributor.authorAslan, Selim
dc.contributor.authorGuzeloglu, Aydin
dc.date.accessioned2020-03-26T18:15:54Z
dc.date.available2020-03-26T18:15:54Z
dc.date.issued2011
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractThe aim was an evaluation of a set of housekeeping genes (HKGs) to be used in the normalization of gene expression in the equine endometrium. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), hypoxanthine ribosyl transferase 1 (HPRT1), ubiquitin B (UBB), tubulin alpha 1 (TUBA1), ribosomal protein L32 (RPL32), beta-2-microglobulin (B2M), 18S rRNA (18S), and 285 rRNA (28S) HKGs were evaluated using real-time PCR and were compared in different physiological stages of the endometrium. Endometrial biopsies were obtained from mares on day of ovulation (d0, n = 4), at late diestrus (LD, n = 4), after luteolyis (AL, n = 4) of the cycle and on days 14 (P14; n = 3), 18 (P18, n = 3) and 22 (P22; n = 3) of pregnancy. A model based on REML with support of descriptive statistics was proposed in accordance with experimental design and was further confirmed with principal component analysis (PCA). Results were compared with widely used software including geNorm, BestKeeper, and NormFinder. Results indicated that GAPDH was the most stable HKG and RPL32 was ranked as the second best. 18S and 285 were found to be the least stable. The proposed model, PCA, geNorm, and BestKeeper were in agreement in detecting the most stable and the least stable HKGs in the equine endometrium during the estrous cycle and early pregnancy. (C) 2011 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipTUBITAKTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [TOVAG 107O035]en_US
dc.description.sponsorshipThis study was financially supported by TUBITAK grant (TOVAG 107O035 to AG). The authors would like to thank Prof. Dr. Sefa Celik for his help in real-time analysis.en_US
dc.identifier.doi10.1016/j.anireprosci.2011.02.019en_US
dc.identifier.endpage132en_US
dc.identifier.issn0378-4320en_US
dc.identifier.issue01.04.2020en_US
dc.identifier.pmid21411251en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage124en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.anireprosci.2011.02.019
dc.identifier.urihttps://hdl.handle.net/20.500.12395/26772
dc.identifier.volume125en_US
dc.identifier.wosWOS:000292668800017en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherELSEVIER SCIENCE BVen_US
dc.relation.ispartofANIMAL REPRODUCTION SCIENCEen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectHousekeeping geneen_US
dc.subjectMixed modelen_US
dc.subjectgeNormen_US
dc.subjectNormFinderen_US
dc.subjectBestKeeperen_US
dc.subjectEquine endometriumen_US
dc.titleRating of putative housekeeping genes for quantitative gene expression analysis in cyclic and early pregnant equine endometriumen_US
dc.typeArticleen_US

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