Comparison of Different Sources of Mesenchymal Stem Cells: Palatal versus Lipoaspirated Adipose Tissue

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dc.contributor.authorHakki, Sema S.
dc.contributor.authorTurac, Gizem
dc.contributor.authorBozkurt, S. Buket
dc.contributor.authorKayis, Seyit Ali
dc.contributor.authorHakki, Erdogan E.
dc.contributor.authorSahin, Eren
dc.contributor.authorSubasi, Cansu
dc.date.accessioned2020-03-26T19:34:27Z
dc.date.available2020-03-26T19:34:27Z
dc.date.issued2017
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractObjectives: The purpose of this study was to compare the proliferation and differentiation potential of mesenchymal stem cells (MSCs) derived from palatal adipose tissue (PAT) and lipoaspirated adipose tissue (LAT). Materials and Methods: PATs were obtained from 2 healthy female patients undergoing surgery for gingival recession, and LATs were obtained from 2 healthy female patients undergoing plastic surgery. LAT-and PAT-derived MSCs were confirmed by flow cytometry using MSC-specific surface markers. The multilineage differentiation capacity of the MSCs was analyzed. The expression of immunophenotyping, embryonic, and differentiation markers was compared between both MSC lines. The proliferation of PAT-and LAT-MSCs was evaluated using a real-time cell analyzer, and telomerase activity was determined using an ELISA-based TRAP assay. Stem cells isolated from PAT and LAT were analyzed by real-time PCR and whole genome array analysis. Results: The cells isolated from PAT had MSC characteristics. In addition, PAT-MSCs had significantly higher alkaline phosphatase activity and osteogenic potential than LAT-MSCs. Although the proliferation and telomerase activities of LAT-MSCs were higher than those of PAT-MSCs, the difference was not statistically significant. The level of embryonic stem cell markers (Oct4 and Nanog) was higher in LAT-MSCs than in PAT-MSCs. The whole genome array analysis demonstrated that 255 gene sequences were differentially expressed, with more than a twofold change in expression. Conclusions: This is the first comparative analysis of the isolation and characterization of MSCs from PAT and LAT. PAT is an accessible source of MSCs, which could be used in periodontal and craniofacial tissue engineering. (C) 2017 S. Karger AG, Baselen_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey, Ankara, TurkeyTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [TUBITAK/SBAG-110S415, TUBITAK/SBAG-112S587]; Selcuk University Research Coordination Office (SELCUK/BAP), Konya, Turkeyen_US
dc.description.sponsorshipThis study was funded by the Scientific and Technological Research Council of Turkey (TUBITAK/SBAG-110S415 and TUBITAK/SBAG-112S587), Ankara, Turkey and the Selcuk University Research Coordination Office (SELCUK/BAP), Konya, Turkey. We thank Dr. Zehra S. Unal for her excellent technical assistance.en_US
dc.identifier.doi10.1159/000478998en_US
dc.identifier.endpage240en_US
dc.identifier.issn1422-6405en_US
dc.identifier.issn1422-6421en_US
dc.identifier.issue05.06.2020en_US
dc.identifier.pmid28848084en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage228en_US
dc.identifier.urihttps://dx.doi.org/10.1159/000478998
dc.identifier.urihttps://hdl.handle.net/20.500.12395/34898
dc.identifier.volume204en_US
dc.identifier.wosWOS:000417977100003en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherKARGERen_US
dc.relation.ispartofCELLS TISSUES ORGANSen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectAdipose tissueen_US
dc.subjectMesenchymal stem cellsen_US
dc.subjectPalatal adipose tissueen_US
dc.subjectLipoaspirationen_US
dc.subjectRegenerative therapiesen_US
dc.titleComparison of Different Sources of Mesenchymal Stem Cells: Palatal versus Lipoaspirated Adipose Tissueen_US
dc.typeArticleen_US

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