Comparison of winter and summer canola (Brassica napus) genotypes in Turkey
Küçük Resim Yok
Tarih
2013
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
FUNPEC-EDITORA
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
We examined genetic relationships between canola (Brassica napus) genotypes cultivated in winter and spring in Turkey. Genomic DNA was isolated from the seeds by two modified CTAB protocols: EZ1 nucleic acid isolation method and a commercial kit (Dneasy Plant Mini Kit, Qiagen). Diversity and genetic relationships in the genotypes were analyzed with RAPD markers; 156 reliable bands were found for both genotypes, of which 24% were polymorphic. Fifteen primers gave at least one consistent polymorphic band. The dendogram developed by pooling data of RAPD analysis of summer and winter genotypes had similar patterns. This technique allowed us to examine the relationship between canola genotypes.
Açıklama
Anahtar Kelimeler
Brassica napus L., Canola, Biodiversity, Genomic DNA, RAPD-PCR
Kaynak
GENETICS AND MOLECULAR RESEARCH
WoS Q Değeri
Q4
Scopus Q Değeri
Q4
Cilt
12
Sayı
1
