Acetylation and methylation of sperm histone 3 lysine 27 (H3K27ac and H3K27me3) are associated with bull fertility

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dc.contributor.authorKutchy, N. A.
dc.contributor.authorMenezes, E. S. B.
dc.contributor.authorChiappetta, A.
dc.contributor.authorTan, W.
dc.contributor.authorWills, R. W.
dc.contributor.authorKaya, A.
dc.contributor.authorTopper, E.
dc.date.accessioned2020-03-26T19:52:48Z
dc.date.available2020-03-26T19:52:48Z
dc.date.issued2018
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractEpigenetic modifications in histones are crucial for proper sperm physiology, egg activation and reproductive development of males. The objectives of this study were to determine the conservation and interactomes of histone three (H3) and ascertain the expression dynamics of acetylated and methylated H3 lysine 27 (H3K27ac and H3K27me3) in spermatozoa from Holstein bulls with different fertility. Methods in immunocytochemistry and flow cytometry were used to evaluate the expression dynamics of H3K27ac and H3K27me3 in spermatozoa from 10 bulls with different in vivo fertility. Computational biology methods including Clustal Omega and Cytoscape were performed to determine the evolutionary conservation and interactome of H3. The post-translational modifications (PTM) of H3 (H3K27ac and H3K27me3) had different spatiotemporal dynamics in the sperm head. Intensities of methylation were higher than those of acetylation and inversely correlated between the two fertility groups (p=.0032). The interacting proteins of H3 are involved in critical subcellular processes such as regulation of methylation, nucleosome assembly, regulation of DNA replication and chromatin assembly. These results are significant because they help advance fundamental science and biotechnology of mammalian reproduction.en_US
dc.description.sponsorshipNetaji Subhas-Indian Council of Agricultural Research, India (ICAR); Mississippi Agricultural and Forestry Experiment Station, MSU; Foundation for Research and Scientific Development of the State of Ceara, Brazil (FUNCAP); Brazilian Research Council (CNPq)National Council for Scientific and Technological Development (CNPq); Brazilian Research Council (CAPES)CAPES; Office of the General Counsel [DBI1262901, EPS 0903787]; Department of Animal and Dairy Sciences, MSUen_US
dc.description.sponsorshipNetaji Subhas-Indian Council of Agricultural Research, India (ICAR).; Mississippi Agricultural and Forestry Experiment Station, MSU; Foundation for Research and Scientific Development of the State of Ceara, Brazil (FUNCAP); Brazilian Research Councils (CNPq and CAPES); Office of the General Counsel, Grant/Award Number: DBI1262901 and EPS 0903787; Department of Animal and Dairy Sciences, MSUen_US
dc.identifier.doi10.1111/and.12915en_US
dc.identifier.issn0303-4569en_US
dc.identifier.issn1439-0272en_US
dc.identifier.issue3en_US
dc.identifier.pmid29057498en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://dx.doi.org/10.1111/and.12915
dc.identifier.urihttps://hdl.handle.net/20.500.12395/36298
dc.identifier.volume50en_US
dc.identifier.wosWOS:000426490200021en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWILEYen_US
dc.relation.ispartofANDROLOGIAen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectacetylationen_US
dc.subjectchromatinen_US
dc.subjectepigeneticsen_US
dc.subjectfertilityen_US
dc.subjectmethylationen_US
dc.subjectspermen_US
dc.titleAcetylation and methylation of sperm histone 3 lysine 27 (H3K27ac and H3K27me3) are associated with bull fertilityen_US
dc.typeArticleen_US

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