Towards multimodal HPLC separations on humic acid-bonded aminopropyl silica: RPLC and LEC behavior

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dc.contributor.authorGezici, Orhan
dc.contributor.authorKara, Huseyin
dc.date.accessioned2020-03-26T18:17:22Z
dc.date.available2020-03-26T18:17:22Z
dc.date.issued2011
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractIn the present study, metal binding property of humic acid (HA) was successfully adapted to the ligand-exchange concept, and metal-loaded immobilized humic acid was used as a ligand exchanger stationary phase for separation of some nucleosides. Humic-acid bonded aminopropyl silica (EC-HA-APS) was turned into ligand exchanger forms by loading aqueous solutions of Cu2+, and Co2+ to the column (4.6 X 150; as mm) packed with EC-HA-APS. Metal ion solutions were loaded to the column in a step-wise manner where the concentration of metal ion solution being loaded to the column was increased gradually between 5 and 100 mM. The progress of metal loading process was monitored via the breakthrough curves propagated stepwise. Ligand-exchange chromatography (LEC) studies were performed on an HPLC system, and chromatographic behaviors of the studied nucleosides (i.e. uridine, Urd; thymidine, Tyd; cytidine, Cyd; adenosine, Ado; and guanosine, Guo) were investigated on Cu2+ and Co2+ loaded forms of the EC-HA-APS (Cu-EC-HA-APS and Co-EC-HA-APS). Effect of mobile phase composition, temperature, and the type of metal ion loaded to the column on the retentive behaviors of the compounds was studied, in detail. The studied solutes exhibited mixed-mode RPLC/LEC behavior on the stationary phase. Metal-loaded column (M-EC-HA-APS) was easily regenerated into its original form. EC-HA-APS, with 98 +/- 2% metal recoveries, by using aqueous mixture of EDTA + NH3 at pH = 7.5. Thus, the stationary phase exhibited a high flexibility between RPLC and LEC modes. This property, also, made it possible to convert the stationary phase into various ligand exchanger forms by loading different metal ions. Hence, capacity and selectivity of the stationary phase towards the studied species was manipulated easily by loading different metal ions to the stationary phase. Baseline separation for the studied species was achieved on Cu-EC-HA-APS and Co-EC-HA-APS and some differentiations were observed in capacity and selectivity, depending on the type of metal loaded. Thus, being as the first endeavor on usability of immobilized HA as a ligand exchanger stationary phase, the present study is believed to be useful to understand multifunctional character of HA-based solid/stationary phases. (C) 2011 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipSelcuk University BAPSelcuk University [082011043]en_US
dc.description.sponsorshipAuthors gratefully acknowledge the financial support provided by Selcuk University BAP. Project Number: 082011043,en_US
dc.identifier.doi10.1016/j.talanta.2011.07.076en_US
dc.identifier.endpage2410en_US
dc.identifier.issn0039-9140en_US
dc.identifier.issn1873-3573en_US
dc.identifier.issue5en_US
dc.identifier.pmid21962660en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage2405en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.talanta.2011.07.076
dc.identifier.urihttps://hdl.handle.net/20.500.12395/27018
dc.identifier.volume85en_US
dc.identifier.wosWOS:000296115600021en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherELSEVIERen_US
dc.relation.ispartofTALANTAen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectHumic aciden_US
dc.subjectImmobilizationen_US
dc.subjectLigand-exchange chromatographyen_US
dc.subjectNucleosidesen_US
dc.subjectSeparationen_US
dc.subjectStationary phaseen_US
dc.titleTowards multimodal HPLC separations on humic acid-bonded aminopropyl silica: RPLC and LEC behavioren_US
dc.typeArticleen_US

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