Exploring the halophyte Cistanche phelypaea (L.) Cout as a source of health promoting products: In vitro antioxidant and enzyme inhibitory properties, metabolomic profile and computational studies

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dc.contributor.authorTrampetti, Francesca
dc.contributor.authorPereira, Catarina
dc.contributor.authorRodrigues, Maria Joao
dc.contributor.authorCelaj, Odeta
dc.contributor.authorD'Abrosca, Brigida
dc.contributor.authorZengin, Gökhan
dc.contributor.authorMollica, Adriano
dc.contributor.authorStefanucci, Azzurra
dc.contributor.authorCustodio, Luisa
dc.date.accessioned2020-03-26T20:14:13Z
dc.date.available2020-03-26T20:14:13Z
dc.date.issued2019
dc.departmentSelçuk Üniversitesi, Fen Fakültesi, Biyoloji Bölümüen_US
dc.description.abstractIn this study, ethyl acetate, acetone, ethanol and water extracts from flowers, stems and roots of Cistanche phelypaea (L.) Cout were appraised for radical scavenging activity (RSA) towards 1,1-diphenyl-2-picrylhydrazyl,2,2-azino-bis(3-ethylbenzo-thiazoline-6-sulfonic acid) and superoxide free radicals, and for metal chelating activities on iron and copper ions. The water extracts had the highest antioxidant activity, especially those from roots and flowers, and were further appraised for in vitro inhibition of enzymes implicated on the onset of human ailments, namely acetyl- (AChE) and butyrylcholinesterase (BuChE) for Alzheimer's disease, alpha-glucosidase and alpha-amylase for diabetes, and tyrosinase for skin hyper-pigmentation disorders. The extracts had a higher activity towards BuChE, and the roots extract had the highest capacity to inhibit tyrosinase. Samples showed a low capacity to inhibit carbohydrate hydrolysing enzymes, except for the root extract with a good inhibition on glucosidase. Samples were then characterized by NMR (1D and 2D): the main metabolites identified in the flowers extract were iridoid glycosides, in particular gluroside and bartsioside. In stems, phenylehanoid glycosides (PhGs) and iri doids were detected, especially acteoside. In roots were detected essentially PhGs, mainly echinacoside and tubuloside A. Docking studies were performed on the identified compounds. A favorable binding energy of tubuloside A to tyrosinase was calculated, and indicated this compound as a possible competitive inhibitor of alpha-glucosidase and tyrosinase. Our results suggest that C. phelypeae is a promising source of biologically-active compounds with health promoting properties for pharmaceutical and biomedical applications. (C) 2018 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipFoundation for Science and Technology (FCT, Portugal)Portuguese Foundation for Science and Technology; Portuguese National Budget funding through the CCMAR project [CCMAR/Multi/04326/2013]; FCTPortuguese Foundation for Science and Technology [IF/00049/2012, SFRH/BD/94407/2013, SFRH/BD/116604/2016]en_US
dc.description.sponsorshipThis work was supported by the Foundation for Science and Technology (FCT, Portugal) and by the Portuguese National Budget funding through the CCMAR/Multi/04326/2013 project. Luisa Custodio was supported by FCT Investigator Programme (IF/00049/2012). Catarina Guerreiro Pereira and Maria Joao Rodrigues acknowledges FCT for the PhD grants SFRH/BD/94407/2013 and SFRH/BD/116604/2016, respectively.en_US
dc.identifier.citationTrampetti, F., Pereira, C., Rodrigues, M. J., Celaj, O., D’Abrosca, B., Zengin, G., Mollica, A., Stedanucci, A., Custódio, L. (2019). Exploring the Halophyte Cistanche phelypaea (L.) Cout as a Source of Health Promoting Products: In Vitro Antioxidant and Enzyme Inhibitory Properties, Metabolomic Profile and Computational Studies. Journal of Pharmaceutical and Biomedical Analysis, 165, 119-128.
dc.identifier.doi10.1016/j.jpba.2018.11.053en_US
dc.identifier.endpage128en_US
dc.identifier.issn0731-7085en_US
dc.identifier.issn1873-264Xen_US
dc.identifier.pmid30529825en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage119en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.jpba.2018.11.053
dc.identifier.urihttps://hdl.handle.net/20.500.12395/37841
dc.identifier.volume165en_US
dc.identifier.wosWOS:000456899500016en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorZengin, Gökhan
dc.language.isoenen_US
dc.publisherELSEVIERen_US
dc.relation.ispartofJOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSISen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectCarbohydrate hydrolysing enzymesen_US
dc.subjectCholinesterasesen_US
dc.subjectComputational approachen_US
dc.subjectOxidative stressen_US
dc.subjectNMRen_US
dc.subjectTyrosinaseen_US
dc.titleExploring the halophyte Cistanche phelypaea (L.) Cout as a source of health promoting products: In vitro antioxidant and enzyme inhibitory properties, metabolomic profile and computational studiesen_US
dc.typeArticleen_US

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