ABM/P-15 modulates proliferation and mRNA synthesis of growth factors of periodontal ligament cells
dc.contributor.author | Emecen, Pinar | |
dc.contributor.author | Akman, Abdullah Cevdet | |
dc.contributor.author | Hakki, Sema Sezgin | |
dc.contributor.author | Hakki, Erdogan Esref | |
dc.contributor.author | Demiralp, Burak | |
dc.contributor.author | Toezeum, Tolga Fikret | |
dc.contributor.author | Nohutcu, Rahime Meral | |
dc.date.accessioned | 2020-03-26T17:37:47Z | |
dc.date.available | 2020-03-26T17:37:47Z | |
dc.date.issued | 2009 | |
dc.department | Selçuk Üniversitesi | en_US |
dc.description.abstract | Objective. Periodontal regeneration is histologically defined as regeneration of the tooth supporting structures, including alveolar bone, periodontal ligament, and cementum. Cells in the remaining periodontal tissues need optimal conditions if they are to perform their functions in the regeneration process. The present study is an investigation of the molecular effects of ABM/P-15 on human periodontal ligament cells (PDL) in vitro. Material and methods. PDL cells obtained from healthy subjects were used for in vitro experiments. Cell proliferation, morphology, and mineralization using Von kossa staining were evaluated. mRNA expressions for transforming growth factor- (TGF-), insulin-like growth factor-I (IGF-I), basic fibroblast growth factor (b-FGF), vascular endothelial growth factor (VEGF), bone morphogenic protein-2 (BMP-2), platelet-derived growth factor (PDGF), and type 1 collagen (COL1) were assessed on days 3 and 7 using RT-PCR. Results. ABM/P-15 enhanced proliferation of cultured PDL cells. It increased the mRNA expression of TGF- and BMP-2 in cultured PDL cells on days 3 and 7. IGF-I and b-FGF mRNA expressions showed a slight decrease, while PDGF expression was observed to have increased on day 3. VEGF and COL1 mRNA expressions were found not to be different on days 3 and 7. No differences were observed in the mineralization properties of cultured PDL cells treated with or without ABM/P-15. Conclusions. Based on the results of this in vitro study, it may be concluded that ABM/P-15 enhanced the regenerative capacity of PDL by regulating specific gene expressions of cells during early wound healing. | en_US |
dc.description.sponsorship | Hacettepe University Research CenterHacettepe University [01.01.201.002] | en_US |
dc.description.sponsorship | The present study was financially supported by the Hacettepe University Research Center (Grant no. 01.01.201.002). Experiments presented in the study were performed at Selcuk University, Research Center of Dental Faculty and Hacettepe University Medical Genetics Application and Research Center. We thank Dr. Burcu Balci of Hacettepe University,Department of Medical Biology, for her kind help in image analyzing. | en_US |
dc.identifier.doi | 10.1080/00016350802555525 | en_US |
dc.identifier.endpage | 73 | en_US |
dc.identifier.issn | 0001-6357 | en_US |
dc.identifier.issn | 1502-3850 | en_US |
dc.identifier.issue | 2 | en_US |
dc.identifier.pmid | 19031159 | en_US |
dc.identifier.scopusquality | Q2 | en_US |
dc.identifier.startpage | 65 | en_US |
dc.identifier.uri | https://dx.doi.org/10.1080/00016350802555525 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12395/23243 | |
dc.identifier.volume | 67 | en_US |
dc.identifier.wos | WOS:000263366500001 | en_US |
dc.identifier.wosquality | Q2 | en_US |
dc.indekslendigikaynak | Web of Science | en_US |
dc.indekslendigikaynak | Scopus | en_US |
dc.indekslendigikaynak | PubMed | en_US |
dc.language.iso | en | en_US |
dc.publisher | TAYLOR & FRANCIS LTD | en_US |
dc.relation.ispartof | ACTA ODONTOLOGICA SCANDINAVICA | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.selcuk | 20240510_oaig | en_US |
dc.subject | ABM | en_US |
dc.subject | P-15 | en_US |
dc.subject | bone-grafting material | en_US |
dc.subject | growth and differentiation factors | en_US |
dc.subject | periodontal ligament cells | en_US |
dc.subject | periodontal regeneration | en_US |
dc.title | ABM/P-15 modulates proliferation and mRNA synthesis of growth factors of periodontal ligament cells | en_US |
dc.type | Article | en_US |