ABM/P-15 modulates proliferation and mRNA synthesis of growth factors of periodontal ligament cells

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dc.contributor.authorEmecen, Pinar
dc.contributor.authorAkman, Abdullah Cevdet
dc.contributor.authorHakki, Sema Sezgin
dc.contributor.authorHakki, Erdogan Esref
dc.contributor.authorDemiralp, Burak
dc.contributor.authorToezeum, Tolga Fikret
dc.contributor.authorNohutcu, Rahime Meral
dc.date.accessioned2020-03-26T17:37:47Z
dc.date.available2020-03-26T17:37:47Z
dc.date.issued2009
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractObjective. Periodontal regeneration is histologically defined as regeneration of the tooth supporting structures, including alveolar bone, periodontal ligament, and cementum. Cells in the remaining periodontal tissues need optimal conditions if they are to perform their functions in the regeneration process. The present study is an investigation of the molecular effects of ABM/P-15 on human periodontal ligament cells (PDL) in vitro. Material and methods. PDL cells obtained from healthy subjects were used for in vitro experiments. Cell proliferation, morphology, and mineralization using Von kossa staining were evaluated. mRNA expressions for transforming growth factor- (TGF-), insulin-like growth factor-I (IGF-I), basic fibroblast growth factor (b-FGF), vascular endothelial growth factor (VEGF), bone morphogenic protein-2 (BMP-2), platelet-derived growth factor (PDGF), and type 1 collagen (COL1) were assessed on days 3 and 7 using RT-PCR. Results. ABM/P-15 enhanced proliferation of cultured PDL cells. It increased the mRNA expression of TGF- and BMP-2 in cultured PDL cells on days 3 and 7. IGF-I and b-FGF mRNA expressions showed a slight decrease, while PDGF expression was observed to have increased on day 3. VEGF and COL1 mRNA expressions were found not to be different on days 3 and 7. No differences were observed in the mineralization properties of cultured PDL cells treated with or without ABM/P-15. Conclusions. Based on the results of this in vitro study, it may be concluded that ABM/P-15 enhanced the regenerative capacity of PDL by regulating specific gene expressions of cells during early wound healing.en_US
dc.description.sponsorshipHacettepe University Research CenterHacettepe University [01.01.201.002]en_US
dc.description.sponsorshipThe present study was financially supported by the Hacettepe University Research Center (Grant no. 01.01.201.002). Experiments presented in the study were performed at Selcuk University, Research Center of Dental Faculty and Hacettepe University Medical Genetics Application and Research Center. We thank Dr. Burcu Balci of Hacettepe University,Department of Medical Biology, for her kind help in image analyzing.en_US
dc.identifier.doi10.1080/00016350802555525en_US
dc.identifier.endpage73en_US
dc.identifier.issn0001-6357en_US
dc.identifier.issn1502-3850en_US
dc.identifier.issue2en_US
dc.identifier.pmid19031159en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage65en_US
dc.identifier.urihttps://dx.doi.org/10.1080/00016350802555525
dc.identifier.urihttps://hdl.handle.net/20.500.12395/23243
dc.identifier.volume67en_US
dc.identifier.wosWOS:000263366500001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTAYLOR & FRANCIS LTDen_US
dc.relation.ispartofACTA ODONTOLOGICA SCANDINAVICAen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectABMen_US
dc.subjectP-15en_US
dc.subjectbone-grafting materialen_US
dc.subjectgrowth and differentiation factorsen_US
dc.subjectperiodontal ligament cellsen_US
dc.subjectperiodontal regenerationen_US
dc.titleABM/P-15 modulates proliferation and mRNA synthesis of growth factors of periodontal ligament cellsen_US
dc.typeArticleen_US

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