Reactions of Some Grapevine Rootstock Cuttings to Mutagenic Applications
Yükleniyor...
Dosyalar
Tarih
2022
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Selçuk Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Grapevine (Vitis spp.) is one of the most important socio-economically important plants in the global scale, and the need for its breeding is increasing. In viticulture, mutation is more promising than crossbreeding in breeding new genotypes from natural genetic diversity. Polyploid plants outperform their diploid relatives in several respects. In this study, the efficacy of oryzalin and N2O mutagens in the induction of polyploidy was investigated by applying different doses and durations to the forced cuttings of 41B [Chasselas (Vitis vinifera L.) × Vitis berlandieri Planch)] and Fercal [(Vitis vinifera x Vitis berlandieri) × 333 EM] rootstocks. LD50 values of mutagen applications were determined, morphological and cytological effects were examined by macroscopic, microscopic, and cytological methods. Application time and dose increase of mutagens decreased LD50 values. As a result of mutagen applications, leaf thickness and chlorophyll content of the surviving plants increased. Applications increased stomatal sizes, decreased their density, increased chloroplast numbers, increased leaf thicknesses and partially SPAD values. It was determined that they were not polyploid in the confirmation test performed with flow cytometry (FC) analyses in 4 Fercal and 1 41B samples that were assumed to be mutant by stoma and chloroplast examinations. After that, it was thought that it would be appropriate to try vegetative material with actively dividing cells, such as nodal cuttings, in the studies of obtaining polyploid individuals on grapevine rootstocks.
Açıklama
Anahtar Kelimeler
41 B, Fercal, Breeding, Mutation, LD50, Polyploidy
Kaynak
Selcuk Journal of Agriculture and Food Sciences
WoS Q Değeri
Scopus Q Değeri
Cilt
36
Sayı
2
Künye
Kara, Z., Doğan, O., (2022). Reactions of Some Grapevine Rootstock Cuttings to Mutagenic Applications. Selcuk Journal of Agriculture and Food Sciences, 36(2), 238-246.
DOI: 10.15316/SJAFS.2022.031
