Reactions of Some Grapevine Rootstock Cuttings to Mutagenic Applications

dc.authorid0000-0003-1096-8288en_US
dc.authorid0000-0002-3264-5925en_US
dc.contributor.authorKara, Zeki
dc.contributor.authorDoğan, Osman
dc.date.accessioned2023-08-06T11:12:59Z
dc.date.available2023-08-06T11:12:59Z
dc.date.issued2022en_US
dc.departmentSelçuk Üniversitesi, Ziraat Fakültesi, Bahçe Bitkileri Bölümüen_US
dc.description.abstractGrapevine (Vitis spp.) is one of the most important socio-economically important plants in the global scale, and the need for its breeding is increasing. In viticulture, mutation is more promising than crossbreeding in breeding new genotypes from natural genetic diversity. Polyploid plants outperform their diploid relatives in several respects. In this study, the efficacy of oryzalin and N2O mutagens in the induction of polyploidy was investigated by applying different doses and durations to the forced cuttings of 41B [Chasselas (Vitis vinifera L.) × Vitis berlandieri Planch)] and Fercal [(Vitis vinifera x Vitis berlandieri) × 333 EM] rootstocks. LD50 values of mutagen applications were determined, morphological and cytological effects were examined by macroscopic, microscopic, and cytological methods. Application time and dose increase of mutagens decreased LD50 values. As a result of mutagen applications, leaf thickness and chlorophyll content of the surviving plants increased. Applications increased stomatal sizes, decreased their density, increased chloroplast numbers, increased leaf thicknesses and partially SPAD values. It was determined that they were not polyploid in the confirmation test performed with flow cytometry (FC) analyses in 4 Fercal and 1 41B samples that were assumed to be mutant by stoma and chloroplast examinations. After that, it was thought that it would be appropriate to try vegetative material with actively dividing cells, such as nodal cuttings, in the studies of obtaining polyploid individuals on grapevine rootstocks.en_US
dc.identifier.citationKara, Z., Doğan, O., (2022). Reactions of Some Grapevine Rootstock Cuttings to Mutagenic Applications. Selcuk Journal of Agriculture and Food Sciences, 36(2), 238-246. DOI: 10.15316/SJAFS.2022.031en_US
dc.identifier.doi10.15316/SJAFS.2022.031en_US
dc.identifier.endpage246en_US
dc.identifier.issn2458-8377en_US
dc.identifier.issue2en_US
dc.identifier.startpage238en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12395/49210
dc.identifier.volume36en_US
dc.institutionauthorKara, Zeki
dc.institutionauthorDoğan, Osman
dc.language.isoenen_US
dc.publisherSelçuk Üniversitesien_US
dc.relation.ispartofSelcuk Journal of Agriculture and Food Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subject41 Ben_US
dc.subjectFercalen_US
dc.subjectBreedingen_US
dc.subjectMutationen_US
dc.subjectLD50en_US
dc.subjectPolyploidyen_US
dc.titleReactions of Some Grapevine Rootstock Cuttings to Mutagenic Applicationsen_US
dc.typeArticleen_US

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