Molecular detection and characterization of Hepatozoon spp. in dogs from the Central part of Turkey

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dc.contributor.authorAydin, Mehmet Fatih
dc.contributor.authorSevinc, Ferda
dc.contributor.authorSevinc, Mutlu
dc.date.accessioned2020-03-26T19:06:22Z
dc.date.available2020-03-26T19:06:22Z
dc.date.issued2015
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractCanine hepatozoonosis is a tick-borne protozoal disease caused by Hepatozoon spp. Two species of Hepatozoon are currently known to infect dogs as Hepatozoon canis and H. americanum. Although H. canis generally causes a chronic infection with relatively mild clinical alterations compared to H. americanum, infection by H. canis can be life-threatening. The disease is widespread in USA, Africa, Europe, South America, and Asia. To determine the frequency of infection with Hepatozoon spp. in stray dogs from Central Anatolia Region of Turkey, a total of 221 blood samples collected over a three-year period were evaluated by using genus specific Polymerase Chain Reaction (PCR) designed to amplify a fragment of 666 bp located in 18 S rRNA gene of Hepatozoon spp. Eight (3.61%) blood samples were positive for Hepatozoon spp. For the classification of species, all positive PCR products were purified with a PCR purification kit and sequenced. Sequencing results of eight representative amplicons indicated that 6 were 98-99% identical to the sequence of H. canis and the other 2 sequences were 95-97% identical to the sequence of Hepatozoon spp. So it was named Hepatozoon sp. MF. A phylogenetic tree was constructed from the sequences of the tick-borne agents identified previously and in this study using the neighbor-joining method. The nucleotide sequences were compared to the H. canis sequences reported in Turkey using the nucleotide Basic Local Alignment Search Tool (BLAST) program. The results of this study are significant in terms of the presence of a novel canine Hepatozoon genotype. (C) 2015 Elsevier GmbH. All rights reserved.en_US
dc.identifier.doi10.1016/j.ttbdis.2015.03.004en_US
dc.identifier.endpage392en_US
dc.identifier.issn1877-959Xen_US
dc.identifier.issn1877-9603en_US
dc.identifier.issue3en_US
dc.identifier.pmid25800100en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage388en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.ttbdis.2015.03.004
dc.identifier.urihttps://hdl.handle.net/20.500.12395/32334
dc.identifier.volume6en_US
dc.identifier.wosWOS:000354158100028en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherELSEVIER GMBH, URBAN & FISCHER VERLAGen_US
dc.relation.ispartofTICKS AND TICK-BORNE DISEASESen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectHepatozoonen_US
dc.subjectPCRen_US
dc.subjectSequenceen_US
dc.subjectCanineen_US
dc.subjectTurkeyen_US
dc.titleMolecular detection and characterization of Hepatozoon spp. in dogs from the Central part of Turkeyen_US
dc.typeArticleen_US

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