Hepatoprotective and antioxidant potential of Asphodeline lutea (L.) Rchb. roots extract in experimental models in vitro/in vivo

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dc.contributor.authorLazarova, Irina
dc.contributor.authorSimeonova, Rumyana
dc.contributor.authorVitcheva, Vessela
dc.contributor.authorKondeva-Burdina, Magdalena
dc.contributor.authorGevrenova, Reneta
dc.contributor.authorZheleva-Dimitrova, Dimitrina
dc.contributor.authorZengin, Gökhan
dc.date.accessioned2020-03-26T19:24:28Z
dc.date.available2020-03-26T19:24:28Z
dc.date.issued2016
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractThe aim of this study was to investigate the effect of Asphodeline lutea (L.) Rchb. dry root extract (ALE) administered alone and against carbon tetrachloride (CCl4)-induced liver injury in vitro/in vivo. The dried roots of A. lutea were extracted with 70% ethanol and was characterized with HPLC-UV. Hepatoprotective potential was investigated by in vivo/in vitro assays in Wistar rats as well as antioxidant properties. At concentrations ranging from 10 to 200 mg/mL of ALE significant cytotoxic effects on isolated hepatocytes were found. ALE showed some toxicity in Wistar rats discerned by increased ALT (Alanine transaminase), ALP (Alkaline phosphatase) activities and MDA (malondialdehyde) quantity, decreased GSH (reduced glutathione) levels without affecting the activity of the antioxidant enzymes (GPx (Gluthatione peroxidase), GR (Glutathione reductase) and GST (Glutathione-S-transferase activity)). The antioxidant and hepatoprotective potential of ALE was also observed in vitro/in vivo against CCl4-induced liver injury, where ALE normalizes all the examined parameters perturbated by CCl4 administration. In addition, ALE preserved the decreased cytochrome P450 level and EMND (Ethylmorphine-N-Demethylase) activity without affecting AH (Aniline 4-Hydroxylase) activity. ALE is rich in anthraquinones, naphthalenes and caffeic acid. The pro-oxidant effects of ALE could be due to naphthalene and anthraquinone bioactivation pathways involving toxic metabolites. (C) 2016 Elsevier Masson SAS. All rights reserved.en_US
dc.description.sponsorshipMedical Science Council at the Medical Univefrsity-Sofia, Bulgaria [13/2014]en_US
dc.description.sponsorshipThis work was supported by a grant 13/2014 from the Medical Science Council at the Medical Univefrsity-Sofia, Bulgaria.en_US
dc.identifier.doi10.1016/j.biopha.2016.06.023en_US
dc.identifier.endpage78en_US
dc.identifier.issn0753-3322en_US
dc.identifier.issn1950-6007en_US
dc.identifier.pmid27470552en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage70en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.biopha.2016.06.023
dc.identifier.urihttps://hdl.handle.net/20.500.12395/33660
dc.identifier.volume83en_US
dc.identifier.wosWOS:000390433400010en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIERen_US
dc.relation.ispartofBIOMEDICINE & PHARMACOTHERAPYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectAnthraquinonesen_US
dc.subjectAsphodeline luteaen_US
dc.subjectCCl4en_US
dc.subjectCytochrome P450en_US
dc.subjectHepatocytesen_US
dc.subjectOxidative stressen_US
dc.titleHepatoprotective and antioxidant potential of Asphodeline lutea (L.) Rchb. roots extract in experimental models in vitro/in vivoen_US
dc.typeArticleen_US

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