First molecular evidence of ocular transmission of Encephalitozoonosis during the intrauterine period in rabbits

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dc.contributor.authorOzkan, Ozcan
dc.contributor.authorKaragoz, Alper
dc.contributor.authorKocak, Nadir
dc.date.accessioned2020-03-26T20:14:18Z
dc.date.available2020-03-26T20:14:18Z
dc.date.issued2019
dc.departmentSelçuk Üniversitesi, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümüen_US
dc.description.abstractMany reports have been published on the suspected vertical transmission of Encephalitozoon cuniculi; however, prior to 2003, these reports were based on circumstantial evidence, such as histopathological, immunohistochemical, or serological diagnosis of the infection. In 2003, vertical transmission of the parasite was confirmed by detection of E. cuniculi DNA in fetuses with the nested polymerase chain reaction (PCR) technique. However, the passage of the parasite to eyes of fetus during the intrauterine stage still requires verification. In the current study, natively infected with parasite spores female rabbits were mated with non-infected males. All resulting offspring that died before ten postpartum days were investigated using molecular techniques to confirm the intrauterine transmission of the parasite to the offspring' eyes. In total, 119 DNA samples from rabbit offspring tissues were collected from blood, kidney, brain, eye (both eyes were used as single samples), lung, placenta, liver and heart were used for PCR. Parasitic DNA in the eyes of offspring was detected (54%) 6 of 11 naturally seropositive mother rabbits. PCR results were found to be positive for the eyes of 63% (19/30) of the offsprings from seropositive rabbits. Therefore, mother rabbits naturally infected with E. cuniculi showed the molecular presence of the parasite in their offspring' eyes. Sequence analysis confirmed the partial DNA sequence data of E. cuniculi and blast analysis identified the agent as genotype I. These results confirm transmission of E. cuniculi to rabbit offspring' eyes in the intrauterine period. This is the first molecular evidence to show ocular transmission of the infection via an intrauterine route in rabbits.en_US
dc.identifier.citationOzkan, O., Karagoz, A., Kocak, N. (2019). First Molecular Evidence Of Ocular Transmission Of Encephalitozoonosis During The İntrauterine Period İn Rabbits. Parasitology İnternational, 71, 1-4.
dc.identifier.doi10.1016/j.parint.2019.03.006en_US
dc.identifier.endpage4en_US
dc.identifier.issn1383-5769en_US
dc.identifier.pmid30853450en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.parint.2019.03.006
dc.identifier.urihttps://hdl.handle.net/20.500.12395/37855
dc.identifier.volume71en_US
dc.identifier.wosWOS:000469890500001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorKocak, Nadir
dc.language.isoenen_US
dc.publisherELSEVIER IRELAND LTDen_US
dc.relation.ispartofPARASITOLOGY INTERNATIONALen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectEncephalitozoonosisen_US
dc.subjectRabbiten_US
dc.subjectVertical transmissionen_US
dc.subjectEyeen_US
dc.subjectPCRen_US
dc.titleFirst molecular evidence of ocular transmission of Encephalitozoonosis during the intrauterine period in rabbitsen_US
dc.typeArticleen_US

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