Bone morphogenetic protein-2, -6, and -7 differently regulate osteogenic differentiation of human periodontal ligament stem cells

dc.contributor.authorHakki, Sema S.
dc.contributor.authorBozkurt, Buket
dc.contributor.authorHakki, Erdogan E.
dc.contributor.authorKayis, Seyit Ali
dc.contributor.authorTurac, Gizem
dc.contributor.authorYilmaz, Irem
dc.contributor.authorKaraoz, Erdal
dc.date.accessioned2020-03-26T18:49:37Z
dc.date.available2020-03-26T18:49:37Z
dc.date.issued2014
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractThe utility of adult stem cells for bone regeneration may be an attractive alternative in the treatment of extensive injury, congenital malformations, or diseases causing large bone defects. To create an environment that is supportive of bone formation, signals from molecules such as the bone morphogenetic proteins (BMPs) are required to engineer fully viable and functional bone. We therefore determined whether BMP-2, -6, and -7 differentially regulate the (1) proliferation, (2) mineralization, and (3) mRNA expression of bone/mineralized tissue associated genes of human periodontal ligament stem cells (hPDLSCs), which were obtained from periodontal ligament tissue of human impacted third molars. hPDLSCs from six participants were isolated and characterized using histochemical and immunohistochemical methods. A real-time cell analyzer was used to evaluate the effects of BMP-2, -6, and -7 on the proliferation of hPDLSCs. hPDLSCs were treated with Dulbecco's modified Eagle's medium containing different concentrations of BMP-2, -6, and -7 (10, 25, 50, 100 ng/mL) and monitored for 264 hours. After dose-response experiments, 50 and 100 ng/mL concentrations of BMPs were used to measure bone/mineralized tissue-associated gene expression. Type I collagen, bone sialoprotein, osteocalcin, osteopontin, and osteoblastic transcription factor Runx2 mRNA expression of hPDLSCs treated with BMP-2, -6, and -7, were evaluated using quantitative RT-PCR. Biomineralization of hPDLSCs was assessed using von Kossa staining. This study demonstrated that BMPs at various concentrations differently regulate the proliferation, mineralization, and mRNA expression of bone/mineralized tissue associated genes in hPDLSCs. BMPs regulate hPDLSC proliferation in a time and dose-dependent manner when compared to an untreated control group. BMPs induced bone/mineralized tissue-associated gene mRNA expression and biomineralization of hPDLSCs. The most pronounced induction occurred in the BMP-6 group in the biomineralization of the hPDLSCs. Our data suggest that BMP-2, -6, and -7 are potent regulators of hPDLSC gene expression and biomineralization. Employing BMPs with hPDLSCs isolated from periodontal ligament tissues provides a promising strategy for bone tissue engineering. (c) 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 102B: 119-130, 2014.en_US
dc.description.sponsorshipScientific and Technological Research Council of TurkeyTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [TUBITAK/SBAG-110S415]; Selcuk University Research Coordination OfficeSelcuk University [BAP-10401104]en_US
dc.description.sponsorshipContract grant sponsor: The Scientific and Technological Research Council of Turkey; contract grant number: TUBITAK/SBAG-110S415 (to S.S.H.); Contract grant sponsor: Selcuk University Research Coordination Office; contract grant number: BAP-10401104 (to S.S.H.)en_US
dc.identifier.doi10.1002/jbm.b.32988en_US
dc.identifier.endpage130en_US
dc.identifier.issn1552-4973en_US
dc.identifier.issn1552-4981en_US
dc.identifier.issue1en_US
dc.identifier.pmid23853066en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage119en_US
dc.identifier.urihttps://dx.doi.org/10.1002/jbm.b.32988
dc.identifier.urihttps://hdl.handle.net/20.500.12395/30649
dc.identifier.volume102en_US
dc.identifier.wosWOS:000328153100014en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWILEY-BLACKWELLen_US
dc.relation.ispartofJOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALSen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectBMP-2en_US
dc.subjectBMP-6en_US
dc.subjectBMP-7en_US
dc.subjectosteogenic differentiationen_US
dc.subjectperiodontal ligament stem cellsen_US
dc.titleBone morphogenetic protein-2, -6, and -7 differently regulate osteogenic differentiation of human periodontal ligament stem cellsen_US
dc.typeArticleen_US

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