Tannic Acid Inhibits Proliferation, Migration, Invasion of Prostate Cancer and Modulates Drug Metabolizing and Antioxidant Enzymes

Basit Öğe Kaydı
dc.contributor.authorKarakurt, Serdar
dc.contributor.authorAdalı, Orhan
dc.date.accessioned2020-03-26T19:26:44Z
dc.date.available2020-03-26T19:26:44Z
dc.date.issued2016
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractThe aim of this study was to investigate the effects of plant phenolic compound tannic acid (TA) on proliferative, metastatic, invasive properties of prostate cancer (PCa) cells; PC-3 and LNCaP, as well as drug metabolizing and antioxidant enzymes. Characterization of TA was done by using FT-IR and NMR. TA dose dependently inhibited the proliferation of PC-3 and LNCaP cells with IC50 values 35.3 mu M and 29.1 mu M, respectively. Wound healing assay showed that TA significantly inhibited (92.7%) migration of PCa cells (p<0.0001). In addition, TA was found to have anti-invasive potential on PC-3 cells and it inhibited (80.9%, p<0.0001) invasion of PC-3 cells into matrigel. Only 17.8% of PC-3 cells can form colony in the 0.7% agarose after treatment of cells with TA at the IC50 value concentration. Furthermore, flow cytometry analyses with Annexin V-APC and 7-AAD staining demonstrated that TA increases early apoptosis rate of PC-3 cells by 25.8% and LNCaP cells by 20.9%. Besides, Western blot and qRT-PCR analyses also demonstrated that TA regulates protein and mRNA expressions of CYP17A1, CYP3A4, CYP2B6, NQO1, GSTM1 and GSTP1 enzymes. The results obtained from this study show that TA might be a good candidate for combinational therapy and highly effective strategic molecule for reducing the occurrence of PCa.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [114Z488]en_US
dc.description.sponsorshipWe thank Dr. Serkan Erdemir for help in NMR studies. This project was funded by Scientific and Technological Research Council of Turkey (TUBITAK), under grant No. 114Z488.en_US
dc.identifier.doi10.2174/1871520616666151111115809en_US
dc.identifier.endpage789en_US
dc.identifier.issn1871-5206en_US
dc.identifier.issn1875-5992en_US
dc.identifier.issue6en_US
dc.identifier.pmid26555610en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage781en_US
dc.identifier.urihttps://dx.doi.org/10.2174/1871520616666151111115809
dc.identifier.urihttps://hdl.handle.net/20.500.12395/34049
dc.identifier.volume16en_US
dc.identifier.wosWOS:000375138000012en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherBENTHAM SCIENCE PUBL LTDen_US
dc.relation.ispartofANTI-CANCER AGENTS IN MEDICINAL CHEMISTRYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectCytochrome P450en_US
dc.subjectmigrationen_US
dc.subjectinvasionen_US
dc.subjectproliferationen_US
dc.subjectprostate canceren_US
dc.subjecttannic aciden_US
dc.titleTannic Acid Inhibits Proliferation, Migration, Invasion of Prostate Cancer and Modulates Drug Metabolizing and Antioxidant Enzymesen_US
dc.typeArticleen_US

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