Isolation of apigenin from subcritical water extracts: Optimization of the process

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dc.contributor.authorCvetanovic, Aleksandra
dc.contributor.authorSvarc-Gajic, Jaroslava
dc.contributor.authorGasic, Uros
dc.contributor.authorTesic, Zivoslav
dc.contributor.authorZengin, Gökhan
dc.contributor.authorZekovic, Zoran
dc.contributor.authorDurovic, Sasa
dc.date.accessioned2020-03-26T19:41:58Z
dc.date.available2020-03-26T19:41:58Z
dc.date.issued2017
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractApigenin is a phytochemical which has been associated with numerous beneficial health effects. However, due to its low concentration and poor bioavailability, isolation from natural sources is very difficult. Subcritical water (SCW) is an excellent solvent for the extraction of moderately polar and non-polar flavonoids. Carefully balanced moderate hydrolytical potential of SCW was used in this work to liberate apigenin from its bound forms. In this research simultaneous extraction and hydrolysis of apigenin bound forms from chamomile have been performed. Optimal operation parameters for maximum yields of apigenin have been investigated and defined (solvent-to-sample ratio 1:30; agitation rate 3 Hz; pressure 45 bar; temperature 115 degrees C; time 30 min). Apigenin was further isolated in its pure form from water extracts obtained under optimal conditions using column chromatography. The purity of isolated compound was confirmed by spectroscopic analysis. Biological activity of extracts obtained under optimal conditions were determined as well. In order to identify polyphenolic compounds UHPLC-HESI-MS/MS analysis was performed. (C) 2016 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipSerbian Ministry of Education, Science and Technological Development [TR31013, 172017]en_US
dc.description.sponsorshipThe present work was carried out within the projects of the Serbian Ministry of Education, Science and Technological Development, (Projects No. TR31013 and 172017). The authors are grateful to Msc Aleksandar Gajic for technical support and Dr Dusan Adamovic, Institute of Field and Vegetable Crops, Backi Petrovac, Serbia, for his support in supplying plant material.en_US
dc.identifier.doi10.1016/j.supflu.2016.10.012en_US
dc.identifier.endpage42en_US
dc.identifier.issn0896-8446en_US
dc.identifier.issn1872-8162en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage32en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.supflu.2016.10.012
dc.identifier.urihttps://hdl.handle.net/20.500.12395/35244
dc.identifier.volume120en_US
dc.identifier.wosWOS:000391075500004en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherELSEVIER SCIENCE BVen_US
dc.relation.ispartofJOURNAL OF SUPERCRITICAL FLUIDSen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectSubcritical water extractionen_US
dc.subjectApigeninen_US
dc.subjectPhenolsen_US
dc.subjectUHPLC-HESI-MS/MS analysisen_US
dc.subjectExtraction parametersen_US
dc.titleIsolation of apigenin from subcritical water extracts: Optimization of the processen_US
dc.typeArticleen_US

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