Detection of Canine Brucellosis by a Rapid Agglutination Test Using Rhizobium Tropici as Antigen

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dc.contributor.authorUçan, Uçkun Sait
dc.contributor.authorAras, Zeki
dc.contributor.authorZorlutuna, M.
dc.date.accessioned2020-03-26T17:47:47Z
dc.date.available2020-03-26T17:47:47Z
dc.date.issued2010
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractThe Brucella canis infection induces various reproductive disorders in dogs and also in humans. The aim of this study was to evaluate the feasability to use antigens from related germs, such as Rhizobium tropici in serological methods for evidencing circulating anti-Brucella antibodies in dogs, because of the zoonotic risk. For that, brucellosis seroprevalence on 135 dogs stemming from pounds or from the Faculty of Veterinary Medicine of the Konya region was determined with specific serological tests (Modified Micro Plate Agglutination Test (MPAT), 2-Mercaptoethanol Tube Agglutination Test (2Me-TAT) an indirect ELISA (I-ELISA)) using B. cants NCTC 10854 strain antigens and with a non specific rapid slide agglutination test (RSAT) using R. tropici CIAT 899 strain as antigens. The canine brucellosis prevalence was 21.5% with I-ELISA and was weakly higher with the MPAT (25.2%) and the 2ME-TAT (22.2%), the relative performances of these 2 specific tests compared to I-ELISA remaining elevated (Agreements, sensibility and specificity around 90% or more). Dogs from pounds and females were significantly the most infected. By contrast, the proportions of false positive and false negative sera with RSAT were markedly elevated (22.6% and 69.0% respectively) leading to low relative performances compared to the I-ELISA (agreement: 67.4%, sensibility: 31.0% and specificity: 77.4%). Genetic similarity between B. canis and R. tropici was determined 30.8% by RAPD-PCR. These results demonstrated that canine brucellosis is a relatively common infection in the Central Anatolia and that the RSAT using R. tropici as antigens cannot be use for the serological diagnosis of the disease.en_US
dc.identifier.citationUçan, U. S., Aras, Z., Zorlutuna, M., (2010). Detection of Canine Brucellosis by a Rapid Agglutination Test Using Rhizobium Tropici as Antigen. Revue De Medecine Veterinaire, 161(2), 51-56.
dc.identifier.endpage56en_US
dc.identifier.issn0035-1555en_US
dc.identifier.issue2en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage51en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12395/24766
dc.identifier.volume161en_US
dc.identifier.wosWOS:000276433300001en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorUçan, Uçkun Sait
dc.institutionauthorAras, Zeki
dc.institutionauthorZorlutuna, M
dc.language.isoenen_US
dc.publisherEcole Nationale Veterinaire Toulouseen_US
dc.relation.ispartofRevue De Medecine Veterinaireen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectBrucella canisen_US
dc.subjectbrucellosisen_US
dc.subjectRhizobium tropicien_US
dc.subjectI-ELISAen_US
dc.subjectagglutination testsen_US
dc.subjectserologyen_US
dc.subjectdogen_US
dc.titleDetection of Canine Brucellosis by a Rapid Agglutination Test Using Rhizobium Tropici as Antigenen_US
dc.typeArticleen_US

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