Fluorescent labelling of DNA on superparamagnetic nanoparticles by a perylene bisimide derivative for cell imaging

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dc.contributor.authorMaltas, Esra
dc.contributor.authorMalkondu, Sait
dc.contributor.authorUyar, Pembegul
dc.contributor.authorOzmen, Mustafa
dc.date.accessioned2020-03-26T19:06:01Z
dc.date.available2020-03-26T19:06:01Z
dc.date.issued2015
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractN,N'-Bis[tris-(2-aminoethyl) amine]-3,4,9,10-perylenetetracarboxylic diimide (PBI-TRIS), nonfluorescent dye was used to fluorescent labelling of DNA. For this aim, (3-aminopropyl) triethoxysilane (APTS) modified superparamagnetic iron oxide nanopartides (SPIONs) were synthesized to provide a suitable surface for binding of DNA. Amine functionalized nanoparticles showed a high immobilization capacity (82.70%) at 25 mg of nanoparticle concentration for Calf thymus DNA. Binding capacity of PBI-TRIS to DNA-SPION was also found as 1.93 mu M on 25 mg of nanoparticles by using UV-vis spectroscopy. Binding of PBI-TRIS to DNA onto nanoparticles was also characterized by scanning electron microscopy and infrared spectroscopy. The confocal images of PBI-TRIS labelled DNA-SPION and breast cells were taken at 488 and 561.7 nm of excitation wavelengths. Cell image was also compared with a commercial dye, DAPI at 403.7 nm of excitation wavelength. Results showed that PBI-TRIS can be used for cell staining. (C) 2014 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipScientific Research Foundation of Selcuk University (BAP)Selcuk Universityen_US
dc.description.sponsorshipWe would like to thank Scientific Research Foundation of Selcuk University (BAP) for supporting this study.en_US
dc.identifier.doi10.1016/j.msec.2014.11.057en_US
dc.identifier.endpage93en_US
dc.identifier.issn0928-4931en_US
dc.identifier.issn1873-0191en_US
dc.identifier.pmid25579900en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage86en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.msec.2014.11.057
dc.identifier.urihttps://hdl.handle.net/20.500.12395/32172
dc.identifier.volume48en_US
dc.identifier.wosWOS:000348749200012en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherELSEVIER SCIENCE BVen_US
dc.relation.ispartofMATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONSen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectCalf thymus DNAen_US
dc.subjectCell imageen_US
dc.subjectFluorescent labellingen_US
dc.subjectPerylene bisimideen_US
dc.titleFluorescent labelling of DNA on superparamagnetic nanoparticles by a perylene bisimide derivative for cell imagingen_US
dc.typeArticleen_US

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