Determination of antibiotic susceptibility, esbl genes and pulsed-field gel electrophoresis profiles of extended-spectrum beta-lactamase-containing escherichia coli isolates

Basit Öğe Kaydı
dc.contributor.authorTutun, Hidayet
dc.contributor.authorKaragoz, Alper
dc.contributor.authorAltintas, Levent
dc.contributor.authorKocak, Nadir
dc.date.accessioned2020-03-26T20:13:17Z
dc.date.available2020-03-26T20:13:17Z
dc.date.issued2019
dc.departmentSelçuk Üniversitesi, Tıp Fakültesi, Genetik Bölümüen_US
dc.description.abstractThe purpose of this study was to determine the phenotypic antibiotic susceptibility patterns, extended-spectrum beta-lactamase (ESBL) genes and genotypic profiles of ESBL-positive Escherichia coli strains isolated from urine samples obtained from outpatients with urinary tract infection in Turkey. A total of 120 E. coli strains during 2017, 2018, and 2019 (40 patients per year) were examined for antibiotic susceptibility patterns by disc diffusion method, for ESBL genes using PCR and sequencing and for molecular typing by pulsed-field gel electrophoresis (PFGE) method. The isolates were evaluated for their sensitivity to 21 different antibiotics. Four different antimicrobial resistance patterns were determined according to antibiotic susceptibility status of the isolates. The beta-lactamase genes detected in the isolates were CTX-M-15 + OXA-1 (n = 14), CTX-M-15 (n = 24), TEM-1 + CTX-M-15 (n = 52), TEM1 + SHV-12 (n = 6), SHV-12 1 (n = 6), TEM-1 + CTX-M-1 (n = 6), TEM-1 + CTX-M-16 (n = 6) and TEM-1 + CTX-M-9 (n = 6). The CTX-M-15 was the most prevalent ESBL enzyme in the isolates. As a result of PFGE analysis performed by XbaI enzyme restriction process, one major PFGE profile and three main groups (Group I-I-III) were observed. While antibiotic resistance profiles of the strains showed four groups (RI-RII-RIII-RIV), PFGE band profiles showed a major group (90% similarity ratio). High ESBL production and decreased susceptibility to broad-spectrum cephalosporins were observed in E. coli strains. In addition, PFGE analysis showed high clonal similarity among E. coli isolates.en_US
dc.identifier.citationTutun, H., Karagoz, A., Altintas, L. (2019). Determination of Antibiotic Susceptibility, ESBL Genes and Pulsed-field Gel Electrophoresis Profiles of Extended-spectrum beta-lactamase-containing Escherichia Coli isolates. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 66(4), 407-416.
dc.identifier.doi10.33988/auvfd.571632en_US
dc.identifier.endpage416en_US
dc.identifier.issn1300-0861en_US
dc.identifier.issn1308-2817en_US
dc.identifier.issue4en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage407en_US
dc.identifier.urihttps://dx.doi.org/10.33988/auvfd.571632
dc.identifier.urihttps://hdl.handle.net/20.500.12395/37661
dc.identifier.volume66en_US
dc.identifier.wosWOS:000484847800013en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.institutionauthorKocak, Nadir
dc.language.isoenen_US
dc.publisherANKARA UNIV PRESSen_US
dc.relation.ispartofANKARA UNIVERSITESI VETERINER FAKULTESI DERGISIen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectAntimicrobial drug resistanceen_US
dc.subjectESBL genesen_US
dc.subjectEscherichia colien_US
dc.subjectmolecular subtypingen_US
dc.subjectpulsed-field gel electrophoresisen_US
dc.titleDetermination of antibiotic susceptibility, esbl genes and pulsed-field gel electrophoresis profiles of extended-spectrum beta-lactamase-containing escherichia coli isolatesen_US
dc.typeArticleen_US

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim
İsim:
Hidayet TUTUN.pdf
Boyut:
802.18 KB
Biçim:
Adobe Portable Document Format
Açıklama:
Full Text Access
İndir

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu
Tıbbi Genetik/Makale Koleksiyonu
TR-Dizin İndeksli Yayınlar Koleksiyonu