Construction of an Amperometric Cholesterol Biosensor Based on DTP(aryl)aniline Conducting Polymer Bound Cholesterol Oxidase

Küçük Resim Yok

Tarih

2018

Dergi Başlığı

Dergi ISSN

Cilt Başlığı

Yayıncı

WILEY-V C H VERLAG GMBH

Erişim Hakkı

info:eu-repo/semantics/closedAccess

Özet

In this study, an amperometric cholesterol biosensor was constructed based on cholesterol oxidase immobilized on a conducting 4-(4H-dithienol[3,2-b:2,3-d]pyrrole-4)aniline polymer, (DTP(aryl)aniline). Glassy carbon electrodes were covered with P(DTP(aryl)aniline) which is used for the wiring of enzyme to the electrode surface by using electro-polymerization. The electron transfer was successfully made by the bio-catalytic activity and possession of the unique morphology of the polymer allowed efficient immobilization of the cholesterol oxidase enzyme. Analytical performances; linear range, detection limit, limit of quantification and the Michaelis-Menten constant (K-m) of biosensor electrodes were obtained 2.0M-23.7M, 0.27M, 0.82M, 17,81M respectively. Biosensor optimization parameters: optimum pH, optimum temperature, stability test and response time were evaluated. The real sample and recovery studies were also performed in order to show applicability of the biosensing electrodes.

Açıklama

Anahtar Kelimeler

Cholesterol biosensor, Cholesterol Oxidase, DTP Conducting polymer

Kaynak

ELECTROANALYSIS

WoS Q Değeri

Q2

Scopus Q Değeri

Q2

Cilt

30

Sayı

10

Künye