Construction of an Amperometric Cholesterol Biosensor Based on DTP(aryl)aniline Conducting Polymer Bound Cholesterol Oxidase

Basit Öğe Kaydı
dc.contributor.authorCevik, Emre
dc.contributor.authorCerit, Alaaddin
dc.contributor.authorGazel, Nilay
dc.contributor.authorYildiz, Huseyin Bekir
dc.date.accessioned2020-03-26T19:53:13Z
dc.date.available2020-03-26T19:53:13Z
dc.date.issued2018
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractIn this study, an amperometric cholesterol biosensor was constructed based on cholesterol oxidase immobilized on a conducting 4-(4H-dithienol[3,2-b:2,3-d]pyrrole-4)aniline polymer, (DTP(aryl)aniline). Glassy carbon electrodes were covered with P(DTP(aryl)aniline) which is used for the wiring of enzyme to the electrode surface by using electro-polymerization. The electron transfer was successfully made by the bio-catalytic activity and possession of the unique morphology of the polymer allowed efficient immobilization of the cholesterol oxidase enzyme. Analytical performances; linear range, detection limit, limit of quantification and the Michaelis-Menten constant (K-m) of biosensor electrodes were obtained 2.0M-23.7M, 0.27M, 0.82M, 17,81M respectively. Biosensor optimization parameters: optimum pH, optimum temperature, stability test and response time were evaluated. The real sample and recovery studies were also performed in order to show applicability of the biosensing electrodes.en_US
dc.identifier.doi10.1002/elan.201800248en_US
dc.identifier.endpage2453en_US
dc.identifier.issn1040-0397en_US
dc.identifier.issn1521-4109en_US
dc.identifier.issue10en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage2445en_US
dc.identifier.urihttps://dx.doi.org/10.1002/elan.201800248
dc.identifier.urihttps://hdl.handle.net/20.500.12395/36442
dc.identifier.volume30en_US
dc.identifier.wosWOS:000446660400031en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherWILEY-V C H VERLAG GMBHen_US
dc.relation.ispartofELECTROANALYSISen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectCholesterol biosensoren_US
dc.subjectCholesterol Oxidaseen_US
dc.subjectDTP Conducting polymeren_US
dc.titleConstruction of an Amperometric Cholesterol Biosensor Based on DTP(aryl)aniline Conducting Polymer Bound Cholesterol Oxidaseen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu