The protective effect of prophylactic ozone administration against retinal ischemia-reperfusion injury

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dc.contributor.authorKal, Ali
dc.contributor.authorKal, Öznur
dc.contributor.authorAkıllıoğlu, İshak
dc.contributor.authorÇelik, Esin
dc.contributor.authorYılmaz, Mustafa
dc.contributor.authorGönül, Şaban
dc.contributor.authorSolmaz, Merve
dc.date.accessioned2020-03-26T19:43:05Z
dc.date.available2020-03-26T19:43:05Z
dc.date.issued2017
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractIntroduction: Retinal ischemia-reperfusion (IR) injury is associated with many ocular diseases. Retinal IR injury leads to the death of retinal ganglion cells (RGCs), loss of retinal function and ultimately vision loss. The aim of this study was to show the protective effects of prophylactic ozone administration against retinal IR injury.Materials and methods: A sham group (S) (n=7) was administered physiological saline (PS) intraperitoneally (i.p.) for 7 d. An ischemia reperfusion (IR) group (n=7) was subjected to retinal ischemia followed by reperfusion for 2h. An ozone group (O) (n=7) was administered 1mg/kg of ozone i.p. for 7 d. In the ozone+IR (O+IR) group (n=7), 1mg/kg of ozone was administered i.p. for 7 d before the IR procedure and at 8 d, the IR injury was created (as in IR group). The rats were anesthetized after second hour of reperfusion and their intracardiac blood was drawn completely and they were sacrificed. Blood samples were sent to a laboratory for analysis of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), malondialdehyde (MDA), total oxidant score (TOS) and total antioxidant capacity (TAC). The degree of retinal injury was evaluated according to changes in retinal cells and necrotic and apoptotic cells using the TUNEL method. Data were evaluated statistically with the Kruskal-Wallis test.Results: The number of RGCs and the inner retinal thickness were significantly decreased after ischemia, and treatment with ozone significantly inhibited retinal ischemic injury. In the IR group, the degree of retinal injury was found to be the highest. In the O+IR group, retinal injury was found to be decreased in comparison to the IR group. In the ozone group without retinal IR injury, the retinal injury score was the lowest. The differences in the antioxidant parameters SOD, GSH-Px and TAC were increased in the ozone group and the lowest in the IR group. The oxidant parameters MDA and TOS were found to be the highest in the IR group and decreased in the ozone group.Discussion: IR injury is also positively correlated with the degree of early apoptosis. This study demonstrated that ozone can attenuate subsequent ischemic damage in the rat retina through triggering the increase of the antioxidant capacity.en_US
dc.identifier.doi10.3109/15569527.2016.1156120en_US
dc.identifier.endpage47en_US
dc.identifier.issn1556-9527en_US
dc.identifier.issn1556-9535en_US
dc.identifier.issue1en_US
dc.identifier.pmid27028056en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage39en_US
dc.identifier.urihttps://dx.doi.org/10.3109/15569527.2016.1156120
dc.identifier.urihttps://hdl.handle.net/20.500.12395/35598
dc.identifier.volume36en_US
dc.identifier.wosWOS:000393946800009en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTAYLOR & FRANCIS LTDen_US
dc.relation.ispartofCUTANEOUS AND OCULAR TOXICOLOGYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectAntioxidanten_US
dc.subjectapoptosisen_US
dc.subjectischemia reperfusion injuryen_US
dc.subjectozoneen_US
dc.subjectretinaen_US
dc.titleThe protective effect of prophylactic ozone administration against retinal ischemia-reperfusion injuryen_US
dc.typeArticleen_US

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