Pomc, inos, pges, il-4, il-5 and il-10 gene expression in peripheral blood mononuclear cells of cyclic and pregnant mares

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dc.contributor.authorKurar, Ercan
dc.contributor.authorAtli, Mehmet Osman
dc.contributor.authorGuzeloglu, Aydin
dc.contributor.authorSemacan, Ahmet
dc.date.accessioned2020-03-26T18:15:40Z
dc.date.available2020-03-26T18:15:40Z
dc.date.issued2011
dc.departmentSelçuk Üniversitesi, Veteriner Fakültesi, Zootekni ve Hayvan Besleme Bölümüen_US
dc.description.abstractAlthough a number of different clinical and laboratory methods are used, there is still need for a practical, reliable and economical diagnosis method for early pregnancy in mares. Objective was to provide a preliminary background for understanding immunological modifications at molecular level during early pregnancy in mare and thereby to allow development of a practical pregnancy test. Blood samples were collected from 10 pregnant and 4 cyclic mares on days of ovulation (d0), 4 and 8. Total RNA samples were isolated from peripheral blood mononuclear cells (PBMC) and cDNA synthesis was performed. Pregnancy dependent gene expression profiles of proopiomelanocortin (POMC), nitric oxide synthase (iNOS), prostaglandin E synthase (PGES), interleukin-4 (IL-4), IL-5 and IL-10 were evaluated at mRNA level using semiquantitative Reverse Transcriptase-Polymerase Chain Reaction. Data were analyzed by General Linear Model and possible differences between all mean factors were determined by Tukey's analysis. No gene expressions were observed for PGES, IL-5 and iNOS in PMBCs. Expressions of POMC, IL-4 and IL-10 were not significant on d0, 4 and 8, which suggested that pregnancy or cyclic status had no effect on expression of these genes. Only, IL-10 mRNA expression was lower in pregnant mares than cyclic mares (P < 0.05).en_US
dc.description.sponsorshipTUBITAK-TOVAGTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [105 O 652]en_US
dc.description.sponsorshipThis study was supported by TUBITAK-TOVAG (105 O 652)en_US
dc.identifier.citationKurar, E., Atlı, M. O., Güzeloğlu, A., Semacan, A. (2011). POMC, iNOS, PGES, IL-4, IL-5 and IL-10 Gene Expression in Peripheral Blood Mononuclear Cells of Cyclic and Pregnant Mares. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 17(2), 319-323.
dc.identifier.endpage323en_US
dc.identifier.issn1300-6045en_US
dc.identifier.issue2en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage319en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12395/26729
dc.identifier.volume17en_US
dc.identifier.wosWOS:000288117600026en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.institutionauthorKurar, Ercan
dc.institutionauthorAtli, Mehmet Osman
dc.institutionauthorGuzeloglu, Aydin
dc.institutionauthorSemacan, Ahmet
dc.language.isoenen_US
dc.publisherKAFKAS UNIV, VETERINER FAKULTESI DERGISIen_US
dc.relation.ispartofKAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISIen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectHorseen_US
dc.subjectPregnancyen_US
dc.subjectPBMCen_US
dc.subjectGene expressionen_US
dc.subjectRT-PCRen_US
dc.titlePomc, inos, pges, il-4, il-5 and il-10 gene expression in peripheral blood mononuclear cells of cyclic and pregnant maresen_US
dc.typeArticleen_US

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