Inhibition and eradication of Salmonella Typhimurium biofilm using P22 bacteriophage, EDTA and nisin
Küçük Resim Yok
Tarih
2018
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Taylor and Francis Ltd.
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
P22 phage >10 5 PFU ml ?1 could be used to inhibit Salmonella Typhimurium biofilm formation by 55–80%. Concentrations of EDTA >1.25 mM and concentrations of nisin >1,200 µg ml ?1 were also highly effective in reducing S. Typhimurium biofilm formation (?96% and ?95% reductions were observed, respectively). A synergistic effect was observed when EDTA and nisin were combined whereas P22 phage in combination with nisin had no synergistic impact on biofilm formation. Triple combination of P22 phage, EDTA and nisin could be also used to inhibit biofilm formation (?93.2%) at a low phage titer (10 2 PFU ml ?1 ), and low EDTA (1.25 mM) and nisin (9.375 µg ml ?1 ) concentrations. A reduction of 70% in the mature biofilm was possible when 10 7 PFU ml ?1 of P22 phage, 20 mM of EDTA and 150 ?g ml ?1 of nisin were used in combination. This study revealed that it could be possible to reduce biofilm formation by S. Typhimurium by the use of P22 phage, EDTA and nisin, either alone or in combination. Although, removal of the mature biofilm was more difficult, the triple combination could be successfully used for mature biofilm of S. Typhimurium. © 2018, © 2018 Informa UK Limited, trading as Taylor & Francis Group.
Açıklama
Anahtar Kelimeler
biofilm, biofilm eradication, biofilm inhibition, EDTA, nisin, P22 bacteriophage, Salmonella Typhimurium
Kaynak
Biofouling
WoS Q Değeri
Scopus Q Değeri
Q2
Cilt
34
Sayı
9
