Inhibition and eradication of Salmonella Typhimurium biofilm using P22 bacteriophage, EDTA and nisin

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dc.contributor.authorYüksel F.N.
dc.contributor.authorBuzrul S.
dc.contributor.authorAkçelik M.
dc.contributor.authorAkçelik N.
dc.date.accessioned2020-03-26T20:11:47Z
dc.date.available2020-03-26T20:11:47Z
dc.date.issued2018
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractP22 phage >10 5 PFU ml ?1 could be used to inhibit Salmonella Typhimurium biofilm formation by 55–80%. Concentrations of EDTA >1.25 mM and concentrations of nisin >1,200 µg ml ?1 were also highly effective in reducing S. Typhimurium biofilm formation (?96% and ?95% reductions were observed, respectively). A synergistic effect was observed when EDTA and nisin were combined whereas P22 phage in combination with nisin had no synergistic impact on biofilm formation. Triple combination of P22 phage, EDTA and nisin could be also used to inhibit biofilm formation (?93.2%) at a low phage titer (10 2 PFU ml ?1 ), and low EDTA (1.25 mM) and nisin (9.375 µg ml ?1 ) concentrations. A reduction of 70% in the mature biofilm was possible when 10 7 PFU ml ?1 of P22 phage, 20 mM of EDTA and 150 ?g ml ?1 of nisin were used in combination. This study revealed that it could be possible to reduce biofilm formation by S. Typhimurium by the use of P22 phage, EDTA and nisin, either alone or in combination. Although, removal of the mature biofilm was more difficult, the triple combination could be successfully used for mature biofilm of S. Typhimurium. © 2018, © 2018 Informa UK Limited, trading as Taylor & Francis Group.en_US
dc.identifier.doi10.1080/08927014.2018.1538412en_US
dc.identifier.endpage1054en_US
dc.identifier.issn0892-7014en_US
dc.identifier.issue9en_US
dc.identifier.pmid30621457en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1046en_US
dc.identifier.urihttps://dx.doi.org/10.1080/08927014.2018.1538412
dc.identifier.urihttps://hdl.handle.net/20.500.12395/37208
dc.identifier.volume34en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTaylor and Francis Ltd.en_US
dc.relation.ispartofBiofoulingen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectbiofilmen_US
dc.subjectbiofilm eradicationen_US
dc.subjectbiofilm inhibitionen_US
dc.subjectEDTAen_US
dc.subjectnisinen_US
dc.subjectP22 bacteriophageen_US
dc.subjectSalmonella Typhimuriumen_US
dc.titleInhibition and eradication of Salmonella Typhimurium biofilm using P22 bacteriophage, EDTA and nisinen_US
dc.typeArticleen_US

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