In vitro screening for antiviral activity of Turkish plants revealing methanolic extract of Rindera lanata var. lanata active against human rotavirus

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dc.contributor.authorCivra, Andrea
dc.contributor.authorFrancese, Rachele
dc.contributor.authorSinato, Davide
dc.contributor.authorDonalisio, Manuela
dc.contributor.authorCagno, Valeria
dc.contributor.authorRubiolo, Patrizia
dc.contributor.authorCeylan, Ramazan
dc.date.accessioned2020-03-26T19:41:53Z
dc.date.available2020-03-26T19:41:53Z
dc.date.issued2017
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractBackground: Human rotavirus (HRoV) is the leading cause of severe gastroenteritis in infants and children under the age of five years. No specific antiviral drug is available for HRoV infections and the treatment of viral diarrhea is mainly based on rehydration and zinc treatment. In this study, we explored medicinal plants endemic to Turkey flora as a source of anti-HRoV compunds. Methods: We performed an antiviral screening on Ballota macrodonta, Salvia cryptantha and Rindera lanata extracts by focus reduction assay. The extract with the highest selectivity index (SI) was selected; its antiviral activity was further confirmed against other HRoV strains and by virus yield reduction assay. The step of viral replicative cycle putatively inhibited was investigated by in vitro assays. Results: The methanolic extract of R. lanata (Boraginaceae) showed the most favourable selectivity index. This extract exhibited a dose-dependent inhibitory activity against three different HRoV strains (EC50 values ranging from 5.8 mu g/ml to 25.5 mu g/ml), but was inactive or barely active against other RNA viruses, namely human rhinovirus and respiratory syncytial virus. The R. lanata extract targets the early steps of HRoV infection, likely by hampering virus penetration into the cells. Conclusion: These results make the R. lanata methanolic extract a promising starting material for a bioguidedfractionation aimed at identifying anti-HRoV compounds. Further work is required to isolate the active principle and assess its clinical potential.en_US
dc.identifier.doi10.1186/s12906-017-1560-3en_US
dc.identifier.issn1472-6882en_US
dc.identifier.pmid28118832en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.urihttps://dx.doi.org/10.1186/s12906-017-1560-3
dc.identifier.urihttps://hdl.handle.net/20.500.12395/35200
dc.identifier.volume17en_US
dc.identifier.wosWOS:000392998500002en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherBIOMED CENTRAL LTDen_US
dc.relation.ispartofBMC COMPLEMENTARY AND ALTERNATIVE MEDICINEen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.titleIn vitro screening for antiviral activity of Turkish plants revealing methanolic extract of Rindera lanata var. lanata active against human rotavirusen_US
dc.typeArticleen_US

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