Multiple pharmacological targets, cytotoxicity, and phytochemical profile of Aphloia theiformis (Vahl.) Benn

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dc.contributor.authorPicot, Marie Carene Nancy
dc.contributor.authorBender, Onur
dc.contributor.authorAtalay, Arzu
dc.contributor.authorZengin, Gökhan
dc.contributor.authorLoffredo, Loic
dc.contributor.authorHadji-Minaglou, Francis
dc.contributor.authorMahomoodally, Mohamad Fawzi
dc.date.accessioned2020-03-26T19:42:07Z
dc.date.available2020-03-26T19:42:07Z
dc.date.issued2017
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractAphloia theiformis (Vahl.) Benn. (AT) is traditionally used in Sub-Saharan African countries including Mauritius as a biomedicine for the management of several diseases. However, there is a dearth of experimental studies to validate these claims. We endeavoured to evaluate the inhibitory effects of crude aqueous extract as traditionally used together with the crude methanol extracts of AT leaves on urease, angiotensin (I) converting enzyme (ACE), acetylcholinesterase (AChE), cholesterol esterase (CEase), glycogen phosphorylase a (GPa), and glycation in vitro. The crude extract showing potent activity against the studied enzymes was further partitioned using different solvents of increasing polarity. The enzyme inhibitory and antiglycation activities of each fraction was assessed. Kinetic of inhibition of the active crude extract/fractions on the aforementioned enzymes was consequently determined using Line-weaver- Burk plots. An ultra-high performance liquid chromatography (UHPLC-UV/MS) system was used to establish the phytochemical profile of AT. The real time cell analysis system (iCELLigence (TM)) was used to monitor any cellular cytotoxicity of AT. Crude methanolextract (CME) was a potent inhibitor of the studied enzymes, with IC50 ranging from 696.22 to 19.73 mu g/mL. CME (82.5%) significantly (p < 0.05) inhibited glycation and was comparable to aminoguanidine (81.5%). Ethyl acetate and n-butanol fractions of CME showed non-competitive, competitive, and uncompetitive mode of inhibition against ACE, CEase, and AChE respectively. Mangiferin, a xanthone glucoside was present in CME, ethyl acetate, and n-butanol fractions. Active extract/fractions were found to be non-cytotoxic (IC50 > 20 mu g/mL) according to the U.S National Cancer Institute plant screening program. This study has established baseline data that tend to justify the traditional use of AT and open new avenues for future biomedicine development. (C) 2017 Elsevier Masson SAS. All rights reserved.en_US
dc.description.sponsorshipTertiary Education Commission (TEC) of Mauritiusen_US
dc.description.sponsorshipThe authors are grateful to the BotaniCert, University of Mauritius, and Ankara University for providing laboratory facilities. This work was financially supported by the Tertiary Education Commission (TEC) of Mauritius. Special thanks to staff of the MontVert Nature Reserve for their help with sample collection.en_US
dc.identifier.doi10.1016/j.biopha.2017.02.031en_US
dc.identifier.endpage350en_US
dc.identifier.issn0753-3322en_US
dc.identifier.issn1950-6007en_US
dc.identifier.pmid28242543en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage342en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.biopha.2017.02.031
dc.identifier.urihttps://hdl.handle.net/20.500.12395/35310
dc.identifier.volume89en_US
dc.identifier.wosWOS:000402468200041en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIERen_US
dc.relation.ispartofBIOMEDICINE & PHARMACOTHERAPYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectAphloia theiformisen_US
dc.subjectMauritiusen_US
dc.subjectCytotoxicityen_US
dc.subjectAntiglycationen_US
dc.subjectMedicinal planten_US
dc.titleMultiple pharmacological targets, cytotoxicity, and phytochemical profile of Aphloia theiformis (Vahl.) Bennen_US
dc.typeArticleen_US

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