Immunohistochemical analysis of orbital connective tissue specimens of patients with active graves ophthalmopathy

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dc.contributor.authorAvunduk, Avni Murat
dc.contributor.authorAvunduk, Mustafa Cihat
dc.contributor.authorPazarlı, Halit
dc.contributor.authorOğuz, Velittin
dc.contributor.authorVarnell, Emily D.
dc.contributor.authorKaufman, Herbert E.
dc.contributor.authorAksoy, Figen
dc.date.accessioned2020-03-26T16:57:20Z
dc.date.available2020-03-26T16:57:20Z
dc.date.issued2005
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractPurpose: To explore the immune mechanism of Graves ophthalmopathy (GO) by analyzing infiltrating cells in orbital connective tissue (OCT) specimens of patients with active GO using immunohistochemical methods. Methods: Five OCT specimens obtained from patients with active GO and five control specimens obtained from forensic cadavers who died from nonmedical reasons were stained with anti-CD3, CD4, CD8, CD45RO, HLA-Dr, CD25, and TNF-alpha monoclonal antibodies. Positively stained cells were counted and results were interpreted as cell counts/mm(2). Four of five GO patients had never been treated with any immunomodulating therapy. Only one had received oral prednisolone prior to tissue sampling, but this treatment had ceased 5 months before surgery. Results: The retro-orbital tissue specimens obtained from forensic cadavers did not show any significant positive staining for any monoclonal antibody tested. However, the specimens from GO patients showed positively stained means of 36.66 +/- 4.61 HLA- Dr(+), 12.8 +/- 3.42 CD8(+), 11.8 +/- 1.78 CD4(+), 16.6 +/- 1.81 CD3(+), 21.2 +/- 3.12 CD45RO(+), 10.4 +/- 2.07 TNF-alpha(+), 7.2 +/- 1.48 CD25(+), 3.2 +/- 1.09 CD4(+) CD8(+), 4.6 +/- 1.67 CD4(+) CD45RO(+), 2.8 +/- 0.83 CD8(+) CD45RO(+), 1.6 +/- 0.89 CD4(+) CD25(+), and 1.8 +/- 1 0.83 CD8(+) CD25(+) cells/ mm(2). Conclusions: Our study supports that most of the infiltrating lymphocytic cells in the active stage of GO are T cells, and a significant proportion of them are CD45RO+ cells. Infiltration of OCT by HLA- Dr+, CD25+, and TNF-alpha cells suggests that Th1-type immune reaction with the interference of proinflammatory cytokine(s) (TNF-alpha) may be important in the pathogenesis of disease. Further studies are needed to understand the disease pathogenesis and may provide a scientific basis for future treatment alternatives for the disease (e. g., anticytokine treatment).en_US
dc.description.sponsorshipNEI NIH HHSUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Eye Institute (NEI) [EY02377]en_US
dc.identifier.doi10.1080/02713680591005931en_US
dc.identifier.endpage638en_US
dc.identifier.issn0271-3683en_US
dc.identifier.issue8en_US
dc.identifier.pmid16109642en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage631en_US
dc.identifier.urihttps://dx.doi.org/10.1080/02713680591005931
dc.identifier.urihttps://hdl.handle.net/20.500.12395/19765
dc.identifier.volume30en_US
dc.identifier.wosWOS:000231977300003en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTAYLOR & FRANCIS INCen_US
dc.relation.ispartofCURRENT EYE RESEARCHen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectCD3en_US
dc.subjectCD4en_US
dc.subjectgraves ophthalmopathyen_US
dc.subjectimmunohistochemistryen_US
dc.subjectTNF-alphaen_US
dc.titleImmunohistochemical analysis of orbital connective tissue specimens of patients with active graves ophthalmopathyen_US
dc.typeArticleen_US

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