p53 expression and apoptosis in liver and spleen during CO2 pneumoperitoneum

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dc.contributor.authorArikan, Yueksel
dc.contributor.authorTosun, Murat
dc.contributor.authorSaykol, Volkan
dc.contributor.authorKalkan, Serpil
dc.contributor.authorErdem, Serpil
dc.date.accessioned2020-03-26T17:27:24Z
dc.date.available2020-03-26T17:27:24Z
dc.date.issued2008
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractBackgrounds and aims Laparoscopic surgery techniques have been increasingly preferred to classic laparotomy by surgeons since 1987. However, this method has some important adverse effects on intra-abdominal organs. The aim of this study is to evaluate the effects of different pressures of CO2 on apoptosis and p53 expression in cells in liver and spleen. Methods In total, 30 male Sprague-Dawley rats were used in the study. CO2 was insufflated into the intra-abdominal cavity via angiocatheter cannule by an insufflator in two different pressures of 10 and 20 mm Hg for 60 min. However, in the control group, only cannule was inserted into the intra-abdominal cavity, but no gas was insufflated. After 60 min, the rats were killed and laparotomy was applied. The liver and spleen were excised. The samples were histologically processed and immunohistochemistry was applied. Results All the data revealed that the number of apoptotic cells in liver and spleen increases in proportion to CO2 pressure level. No p53 expression was detected in both organs. Conclusion CO2 pressure level and application time may affect on cells living in liver and spleen. High pressure and/or long application time may cause releasing of cytokines and superoxide radicals from these organs' cells, and transient or serious organ dysfunctions may occur.en_US
dc.description.sponsorshipAfyon Kocatepe University Scientific Research Projects CommissionAfyon Kocatepe University [051TIP-10]en_US
dc.description.sponsorshipThis study has been financed by Afyon Kocatepe University Scientific Research Projects Commission (approval no. 051TIP-10).en_US
dc.identifier.doi10.1007/s00423-007-0254-6en_US
dc.identifier.endpage882en_US
dc.identifier.issn1435-2443en_US
dc.identifier.issue6en_US
dc.identifier.pmid18057955en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage877en_US
dc.identifier.urihttps://dx.doi.org/10.1007/s00423-007-0254-6
dc.identifier.urihttps://hdl.handle.net/20.500.12395/22554
dc.identifier.volume393en_US
dc.identifier.wosWOS:000259819300008en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSPRINGERen_US
dc.relation.ispartofLANGENBECKS ARCHIVES OF SURGERYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectCO2 pneumoperitoneumen_US
dc.subjectapoptosisen_US
dc.subjectp53en_US
dc.subjectliveren_US
dc.subjectspleenen_US
dc.titlep53 expression and apoptosis in liver and spleen during CO2 pneumoperitoneumen_US
dc.typeArticleen_US

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