In vitro antioxidant capacity and phytochemical characterization of Eryngium kotschyi Boiss
Yükleniyor...
Dosyalar
Tarih
2020
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
JOURNAL PHARMACY & PHARMACOGNOSY RESEARCH-JPPRES
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Context: Eryngium kotschyi is one of the species of Eryngium genus and was endemic to Turkey. It was known, that traditionally uses of this plant in the South-Western part of Turkey for the treatment of various diseases. Aims: To evaluate the antioxidant capacities of methanol extract and ethyl acetate, n-butanol and water sub-extracts as well as to determine the phytochemical composition and to quantify the major antioxidant compounds in the active sub-extract. Methods: Besides the total phenolic compounds and total flavonoid compounds, the antioxidant capacity of E. kotschyi was evaluated by DPPH center dot, ABTS+center dot and FRAP methods and qualitative and quantitative determination of phytochemical constituents in active sub-extract by LC-MS/MS. Results: The highest total phenolic (173.710 +/- 1.088 mg gallic acid equivalent/ g(exteact)) and total flavonoid content (86.978 +/- 0.650 mg catechin equivalent/ g(exteact)) was found in ethyl acetate sub-extract of E. kotschyi (EKE) and this sub-extract showed the highest antioxidant capacity on DPPH center dot (IC50 = 0.264 +/- 0.040 mg/ mL), ABTS+center dot (at a concentration of 0.125 mg/mL equivalent to 0.497 mu M Trolox) and FRAP (at a concentration of 1 mg/ mL equivalent to 1476 +/- 5.292 mmol Fe2+) tests. Preliminary examination of the mass spectrums revealed the presence of 24 phytochemical compounds in this sub-extract and 7 of them was quantified. According to quantitative analyses the main compounds of EKE sub-extract were rosmarinic acid (490.820 +/- 0.703 mu g/mgextoet), chlorogenic acid (80.405 +/- 0.170 mu g/mg(exteeet)), isorhamnetin 3-O-rutinoside (72.280 +/- 0.33 mu g/ mgexteeet), rutin (63.020 +/- 0.052 mu g/mg(exteeet)) and caffeic acid (55.153 +/- 0.523 mu g/ mg(exteeet)). Conclusions: The data suggest that EKE possess utilizable antioxidant properties in vitro. The antioxidant capacity of this sub-extract could be due to high content of phenolic and flavonoid contents.
Açıklama
Anahtar Kelimeler
ABTS(+center dot), antioxidant DPPH center dot, Eryngium kotschyi, FRAP, LC-MS/MS
Kaynak
JOURNAL OF PHARMACY & PHARMACOGNOSY RESEARCH
WoS Q Değeri
N/A
Scopus Q Değeri
Cilt
8
Sayı
1
Künye
Paşayeva, L., Şafak, E. K., Arıgün, T., Fatullayev, H., Tugay, O. (2020). In vitro Antioxidant Capacity and Phytochemical Characterization of Eryngium kotschyi Boiss. Journal of Pharmacy & Pharmacognosy Research, 8(1), 18-31.
