In vitro antioxidant capacity and phytochemical characterization of Eryngium kotschyi Boiss

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dc.contributor.authorPasayeva, Leyla.
dc.contributor.authorSafak, Esra Kongul.
dc.contributor.authorArigun, Tunahan.
dc.contributor.authorFatullayev, Hanifa.
dc.contributor.authorTugay, Osman.
dc.date.accessioned2020-03-26T20:20:19Z
dc.date.available2020-03-26T20:20:19Z
dc.date.issued2020
dc.departmentSelçuk Üniversitesi, Eczacılık Fakültesi, Eczacılık Meslek Bilimleri Bölümüen_US
dc.description.abstractContext: Eryngium kotschyi is one of the species of Eryngium genus and was endemic to Turkey. It was known, that traditionally uses of this plant in the South-Western part of Turkey for the treatment of various diseases. Aims: To evaluate the antioxidant capacities of methanol extract and ethyl acetate, n-butanol and water sub-extracts as well as to determine the phytochemical composition and to quantify the major antioxidant compounds in the active sub-extract. Methods: Besides the total phenolic compounds and total flavonoid compounds, the antioxidant capacity of E. kotschyi was evaluated by DPPH center dot, ABTS+center dot and FRAP methods and qualitative and quantitative determination of phytochemical constituents in active sub-extract by LC-MS/MS. Results: The highest total phenolic (173.710 +/- 1.088 mg gallic acid equivalent/ g(exteact)) and total flavonoid content (86.978 +/- 0.650 mg catechin equivalent/ g(exteact)) was found in ethyl acetate sub-extract of E. kotschyi (EKE) and this sub-extract showed the highest antioxidant capacity on DPPH center dot (IC50 = 0.264 +/- 0.040 mg/ mL), ABTS+center dot (at a concentration of 0.125 mg/mL equivalent to 0.497 mu M Trolox) and FRAP (at a concentration of 1 mg/ mL equivalent to 1476 +/- 5.292 mmol Fe2+) tests. Preliminary examination of the mass spectrums revealed the presence of 24 phytochemical compounds in this sub-extract and 7 of them was quantified. According to quantitative analyses the main compounds of EKE sub-extract were rosmarinic acid (490.820 +/- 0.703 mu g/mgextoet), chlorogenic acid (80.405 +/- 0.170 mu g/mg(exteeet)), isorhamnetin 3-O-rutinoside (72.280 +/- 0.33 mu g/ mgexteeet), rutin (63.020 +/- 0.052 mu g/mg(exteeet)) and caffeic acid (55.153 +/- 0.523 mu g/ mg(exteeet)). Conclusions: The data suggest that EKE possess utilizable antioxidant properties in vitro. The antioxidant capacity of this sub-extract could be due to high content of phenolic and flavonoid contents.en_US
dc.description.sponsorshipErciyes University Scientific Research Projects Coordinating Unit (BAP)Erciyes University [TLO-20199411]en_US
dc.description.sponsorshipAuthors thank to Erciyes University Scientific Research Projects Coordinating Unit (BAP, project number TLO-20199411) for financial support and to Erciyes University Drug Application and Research Center (ERFARMA) for LC-MS/MS facility.en_US
dc.identifier.citationPaşayeva, L., Şafak, E. K., Arıgün, T., Fatullayev, H., Tugay, O. (2020). In vitro Antioxidant Capacity and Phytochemical Characterization of Eryngium kotschyi Boiss. Journal of Pharmacy & Pharmacognosy Research, 8(1), 18-31.
dc.identifier.endpage31en_US
dc.identifier.issn0719-4250en_US
dc.identifier.issue1en_US
dc.identifier.pmid#YOKen_US
dc.identifier.startpage18en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12395/38558
dc.identifier.volume8en_US
dc.identifier.wosWOS:000497978000002en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.institutionauthorTugay, Osman.
dc.language.isoenen_US
dc.publisherJOURNAL PHARMACY & PHARMACOGNOSY RESEARCH-JPPRESen_US
dc.relation.ispartofJOURNAL OF PHARMACY & PHARMACOGNOSY RESEARCHen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectABTS(+center dot)en_US
dc.subjectantioxidant DPPH center doten_US
dc.subjectEryngium kotschyien_US
dc.subjectFRAPen_US
dc.subjectLC-MS/MSen_US
dc.titleIn vitro antioxidant capacity and phytochemical characterization of Eryngium kotschyi Boissen_US
dc.typeArticleen_US

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