Isolation Rate of Mycobacterium tuberculosis Complex from Patients with Suspected Tuberculosis and Identification of the Strains with BACTEC (TM) NAP and Immunochromatographic TB Ag MPT64 Rapid (TM) Tests

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dc.contributor.authorKurtoglu, Muhammet Guzel
dc.contributor.authorOzdemir, Mehmet
dc.contributor.authorKesli, Recep
dc.contributor.authorOzkalp, Birol
dc.contributor.authorBaysal, Bulent
dc.date.accessioned2020-03-26T18:15:05Z
dc.date.available2020-03-26T18:15:05Z
dc.date.issued2011
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractTuberculosis (TB) which is still one of the important infectious diseases in the world as well as Turkey, results in high morbidity and mortality. Clinical mycobacteriology laboratories have crucial roles in the identification, typing and susceptibility testing of Mycobacterium tuberculosis. The aims of this study were the investigation of the isolation rate of M.tuberculosis complex (MTC) from the clinical specimens of TB-suspected patients and to compare identification of mycobacteria isolated from solid and/or liquid media by using BACTEC NAP and immunochromatographic TB Ag MPT64 rapid test. A total of 1670 patients who were admitted to outpatients clinics of our hospital and prediagnosed as TB, have been included in the study. All the patients were anti-HIV seronegative. NALC-NaOH method were used for decontamination/homogenization, and preparations from samples were stained with Erlich-Ziehl-Neelsen method to detect acid-resistant bacilli (ARB) in direct microscopy. All of the samples were inoculated into BACTEC (TM) MGIT-960 (Becton Dickinson, USA) and Lowenstein-Jensen (14) media for cultivation and incubated at 37 degrees C for 6-8 weeks. Mycobacteria that were grown in the media have been identified by BACTEC (TM) NAP (Becton Dickinson, USA) and TB Ag MPT64 rapid test (SD Bioline Ag MPT64 Rapid (TM); Standard Diagnostics, Korea). The culture positivity in the samples of TB-suspected patients was found to be 3.7% (63/1670) with LJ and/or MGIT-960 methods, whereas ARB positivity rate was 1.6% (28/1670). Fifty-three (84%) out of culture positive 63 samples have been identified as MTC by BACTEC NAP test, while 61(97%) were found as MTC by TB MPT64 test. Considering BACTEC NAP test as the reference method, TB MPT64 test identified all the MTC strains correctly (sensitivity: 100%), however the false positivity rate was estimated as 12.7% (specificity: 87%). Of 53 MTC positive samples, 36 were sputum, four were bronchoalveolar lavage, four were urine, three were gastric fluid, three were pleural fluid, and one of each were abscess, peritoneal fluid and cerebrospinal fluid samples. ARB positivity rate was detected as 41.5% (22/53) among MTC culture positive samples. Of the patients who were infected with MTC, 72% (38/53) were male and 98% (52/53) were adults (age range: 20-85 years). Our data indicating 3.1% (53/1670) isolation rate of MTC from TB-suspected patients in our region were in concordance with the other results reported from Turkey. In conclusion, immunochromatographic TB Ag MPT64 test which seemed to be useful for the rapid identification of mycobacteria grown on solid and/or liquid, was practical to perform and had high sensitivity, however further larger-scaled studies are needed to support our data in our country.en_US
dc.identifier.endpage273en_US
dc.identifier.issn0374-9096en_US
dc.identifier.issue2en_US
dc.identifier.pmid21644069en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage266en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12395/26601
dc.identifier.volume45en_US
dc.identifier.wosWOS:000291333900007en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isotren_US
dc.publisherANKARA MICROBIOLOGY SOCen_US
dc.relation.ispartofMIKROBIYOLOJI BULTENIen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectTuberculosisen_US
dc.subjectcultureen_US
dc.subjectMycobacterium tuberculosis complexen_US
dc.subjectBACTEC (TM) NAP testen_US
dc.subjectTB Ag MPT64 testen_US
dc.subjectTurkeyen_US
dc.titleIsolation Rate of Mycobacterium tuberculosis Complex from Patients with Suspected Tuberculosis and Identification of the Strains with BACTEC (TM) NAP and Immunochromatographic TB Ag MPT64 Rapid (TM) Testsen_US
dc.typeArticleen_US

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