The effects of different sugars on motility, morphology and DNA damage during the liquid storage of rat epididymal sperm at 4 degrees C

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dc.contributor.authorSariozkan, Serpil
dc.contributor.authorBucak, Mustafa Numan
dc.contributor.authorCanturk, Fazile
dc.contributor.authorOzdamar, Sairn
dc.contributor.authorYay, Arzu
dc.contributor.authorTuncer, Purhan Barbaros
dc.contributor.authorOzcan, Servet
dc.date.accessioned2020-03-26T18:31:40Z
dc.date.available2020-03-26T18:31:40Z
dc.date.issued2012
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractThis study evaluated the protective effects of supplementation with three different sugars on the motility, morphology and DNA integrity of rat epididymal sperm chilled and stored at 4 degrees C Epididymides were obtained from each donor. Rat epididymal sperm was diluted in Ham's F10 plus raffinose, Ham's F10 plus trehalose, Ham's F10 plus fructose, and Ham's F10 medium for control purposes. Thereafter, the extended sperm were chilled and stored in liquid form at 4 degrees C. Sperm motility, morphological abnormalities and DNA damage were determined at 0 and 12 h after chilling. No significant difference was observed in any of the parameters evaluated at 0 h, before storage (P > 0.05). After 12 h of storage, all sugar additives led to statistically higher motility, normal sperm morphology and DNA integrity in comparison to the control group. Raffinose gave the best motility percentages (32.86 +/- 1.84%) after 12 h of storage at 4 degrees C, compared to the other groups (P < 0.001). In conclusion, Raffinose, trehalose and fructose provided a better protection of sperm functional parameters against chilling injury, in comparison to the control group. Crown Copyright (C) 2012 Published by Elsevier Inc. All rights reserved.en_US
dc.identifier.doi10.1016/j.cryobiol.2012.05.007en_US
dc.identifier.endpage97en_US
dc.identifier.issn0011-2240en_US
dc.identifier.issn1090-2392en_US
dc.identifier.issue2en_US
dc.identifier.pmid22634115en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage93en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.cryobiol.2012.05.007
dc.identifier.urihttps://hdl.handle.net/20.500.12395/28508
dc.identifier.volume65en_US
dc.identifier.wosWOS:000307683000003en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherACADEMIC PRESS INC ELSEVIER SCIENCEen_US
dc.relation.ispartofCRYOBIOLOGYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectChillingen_US
dc.subjectRat spermen_US
dc.subjectFructoseen_US
dc.subjectRaffinoseen_US
dc.subjectTrehaloseen_US
dc.titleThe effects of different sugars on motility, morphology and DNA damage during the liquid storage of rat epididymal sperm at 4 degrees Cen_US
dc.typeArticleen_US

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