Early pregnancy-related changes in toll-like receptors expression in ovine trophoblasts and peripheral blood leukocytes

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dc.contributor.authorKaya, Mehmet Salih
dc.contributor.authorKose, Mehmet
dc.contributor.authorGuzeloglu, Aydin
dc.contributor.authorKiyma, Zekeriya
dc.contributor.authorAtli, Mehmet Osman
dc.date.accessioned2020-03-26T19:35:00Z
dc.date.available2020-03-26T19:35:00Z
dc.date.issued2017
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractIn the present study, we aimed to 1) demonstrate the presence of all 10 toll-like receptors (TLRs) in ovine trophoblasts, and 2) investigate the expression profiles of TLR1 - 10 mRNAs in peripheral blood leukocytes (PBLs) in ewes during early pregnancy. For those purposes, ovine trophoblasts (n = 6) were collected from pregnant ewes on day 13. PBLs were collected from non-pregnant (n = 6) and pregnant ewes (n = 17) on days of mating (d) 0 and 18. TLR mRNAs in ovine trophoblasts were visualized by free-floating in situ hybridization (ISH). To assess the expression profiles of TLR1-10 in PBLs, total RNA was isolated and transcribed to cDNA. TLR1-10 mRNA levels were determined by real-time PCR in triplicate. The Relative Expression Software Tool (REST 2009) was used for statistical analysis. We detected mRNAs for TLR2, TLR4, TLR5, TLR6, TLR7, TLR8, and TLR10 but not for TLR1, TLR3, and TLR9 in trophoblasts. TLR2, TLR5, TLR6, TLR7, TLR8, and TLR10 mRNAs were expressed by all trophoblasts, whereas TLR4 mRNA and protein in trophoblasts were more limited. In PBLs, TLR expression did not differ between day 0 and day 18 in non-pregnant ewes; however, ewes in early pregnancy exhibited significantly upregulated expression of TLR2 (23-fold), TLR4 (3.1-fold), TLR6 (1.7-fold), and TLR8 (2.2-fold) on day 18 compared with day 0. In contrast, TLR10 was downregulated (2-fold) on day 18 by pregnancy. Similar results were also obtained for TLR2, TLR4, TLR6, TLR8 and TLR10 from the comparison between day 18 non pregnant and day 18 pregnant groups. According to these results, the presence of TLRs in early ovine trophoblasts suggests that these cells play an immunological role at the maternal fetal interface. The results also suggest that tight regulation of some components of TLRs in PBLs due to embryo- and/or pregnancy related factors is necessary for successful establishment of early pregnancy in ewes. (C) 2017 Elsevier Inc. All rights reserved.en_US
dc.description.sponsorshipDicle University Grant CommissionDicle University [13-VF-123]; TUBITAK (The Scientific and Technological Research Council of Turkey) through Scientific and Technological Research Projects Funding Program [112R022]en_US
dc.description.sponsorshipThis study was supported by Dicle University Grant Commission of research numbered with 13-VF-123 and TUBITAK (The Scientific and Technological Research Council of Turkey) through Scientific and Technological Research Projects Funding Program (Project No: 112R022).en_US
dc.identifier.doi10.1016/j.theriogenology.2017.01.031en_US
dc.identifier.endpage45en_US
dc.identifier.issn0093-691Xen_US
dc.identifier.issn1879-3231en_US
dc.identifier.pmid28257865en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage40en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.theriogenology.2017.01.031
dc.identifier.urihttps://hdl.handle.net/20.500.12395/34987
dc.identifier.volume93en_US
dc.identifier.wosWOS:000396183900007en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherELSEVIER SCIENCE INCen_US
dc.relation.ispartofTHERIOGENOLOGYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectTrophoblastsen_US
dc.subjectPeripheral blood leukocytesen_US
dc.subjectToll-like receptorsen_US
dc.subjectEarly pregnancyen_US
dc.subjectEween_US
dc.titleEarly pregnancy-related changes in toll-like receptors expression in ovine trophoblasts and peripheral blood leukocytesen_US
dc.typeArticleen_US

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