Multi-targeted potential of Pittosporum senacia Putt.: HPLC-ESI-MSn analysis, in silico docking, DNA protection, antimicrobial, enzyme inhibition, anticancer and apoptotic activity
Yükleniyor...
Dosyalar
Tarih
2019
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
ELSEVIER SCI LTD
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Pittosporum senacia (PS) Putt. (Pittosporaceae), indigenous to the Mascarene Islands, is a common ingredient in traditional medicines. However, there is currently a dearth of studies to validate some of these traditional claims. Given the broad traditional uses of PS against several diseases, we aimed to provide a comprehensive insight into the biological and chemical profile of P. senacia. The antioxidant, enzyme inhibitory activity, anticancer, and phytochemical composition of the methanolic extract of P. senacia leaf extracts were studied. The possible interaction and binding mode of the most abundant phytochemicals were studied via in silico docking experiments on tyrosinase and alpha-glucosidase. The mechanism behind the cytotoxic property of P. senacia extract for MDA-MB-231 was also examined using different methods including 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cell viability test checking apoptosis-associated genes, and wound healing assays. Twenty-six compounds were identified, of which caffeoylquinic acid derivatives, ferulic acid derivative, cinnamoylquinic acid derivative and two other polyphenols (oleuropeine and isoramnetin glucoside) being abundant, have been tested using in silico studies, against alpha-glucosidase and tyrosinase. The extract (IC50 = 118.8 mu g/ml) exhibited time and dose dependent anti-proliferative effect on human breast cancer cell line, MDA-MB-231. According to the expression profile of apoptosis inhibitors and apoptosis promoters genes, expression of Bar and Bak genes were significantly increased compared to Bcl-2 and Birc5 genes. Based on wound healing analysis, cell migration was inhibited after the application of the plant extract. The present findings suggested that PS might be a good candidate as sources of bioactive compounds for designing functional applications.
Açıklama
Anahtar Kelimeler
Pittosporaceae, Mauritius, Diabetes, Antimicrobial, Apoptosis
Kaynak
COMPUTATIONAL BIOLOGY AND CHEMISTRY
WoS Q Değeri
Q3
Scopus Q Değeri
Q2
Cilt
83
Sayı
Künye
Mahomoodally, M. F., Picot-Allain, C., Hosenally, M., Ugurlu, A., Mollica, A., Stefanucci, A., Llorent-Martinez, E. J.., Baloglu, M.C., Zengin, G. (2019). Multi-Targeted Potential Of Pittosporum Senacia Putt.: HPLC-ESI-Msn Analysis, İn Silico Docking, DNA Protection, Antimicrobial, Enzyme İnhibition, Anti-Cancer And Apoptotic Activity. Computational Biology And Chemistry, 83, 107114.
