Effect of glutamine and sugars after bull spermatozoa cryopreservation

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dc.contributor.authorTuncer, Purhan Barbaros
dc.contributor.authorSarıözkan, Serpil
dc.contributor.authorBucak, Mustafa Numan
dc.contributor.authorUlutaş, Pınar Alkım
dc.contributor.authorAkalın, Pınar Peker
dc.contributor.authorBüyükleblebici, Serhat
dc.contributor.authorCantürk, Fazile
dc.date.accessioned2020-03-26T18:14:15Z
dc.date.available2020-03-26T18:14:15Z
dc.date.issued2011
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractThe objective of this study was to evaluate the effects of the addition of different sugars (raffinose, sucrose, and trehalose) on bull spermatozoa cryopreserved in a commercial extender (Optidyl) supplemented with glutamine on semen parameters, fertilizing ability and superoxide dismutase (SOD) activity. Nine ejaculates for each bull were used in the study. Semen was frozen in five different extenders: raffinose 25 mM plus glutamine 3 mM (RGO), sucrose 25 mM plus glutamine 3 mM (SGO), trehalose 25 mM plus glutamine 3 mM (TGO), glutamine 3 mM (GO) and control (0). Insemination doses were processed so that each 0.25 mL straw contained 15 x 10(6)sperm. Groups of GO and RGO resulted in the higher rates of subjective (54.0 +/- 1.7% and 64.0 +/- 1.1%; P < 0.01) and CASA motilities (53.0 +/- 2.7% and 61.0 +/- 4.4%; P < 0.001), respectively compared to the other groups. The supplementation of additives did not provide an effect on the level of post-thaw sperm CASA progressive motilities, the sperm motion characteristics and pregnancy rates. GO and RGO provided the better protective effect for sperm acrosome (4.0 +/- 0.5% and 12.0 +/- 0.6%) and total abnormalities (5.0 +/- 0.3% and 13.0 +/- 0.7%; P < 0.001), respectively. At the HOST values, the additives did not give to result the protective effect in comparison to Optydil extender without additives (P > 0.05). For pregnancy rates, there were no significant differences among the groups. The supplementation of additives did not provide any significant difference on the level of SOD activity (P > 0.05). It can be also thought that these sugars might have worked with glutamine in a synergy. Thereby, sugars such as raffinose and sucrose with glutamine in freezing extender may be recommended to facilitate bull semen freezability. (C) 2011 Elsevier Inc. All rights reserved.en_US
dc.description.sponsorshipRepublic of Turkey, Ministry of Agriculture and Rural Affairs, Lalahan Livestock Central Research Institute, Ankara, TurkeyGida Tarim Ve Hayvancilik Bakanligien_US
dc.description.sponsorshipThis study was supported by the Republic of Turkey, Ministry of Agriculture and Rural Affairs, Lalahan Livestock Central Research Institute, Ankara, Turkey.en_US
dc.identifier.doi10.1016/j.theriogenology.2010.12.006en_US
dc.identifier.endpage1465en_US
dc.identifier.issn0093-691Xen_US
dc.identifier.issn1879-3231en_US
dc.identifier.issue8en_US
dc.identifier.pmid21354604en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1459en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.theriogenology.2010.12.006
dc.identifier.urihttps://hdl.handle.net/20.500.12395/26363
dc.identifier.volume75en_US
dc.identifier.wosWOS:000289598300010en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherELSEVIER SCIENCE INCen_US
dc.relation.ispartofTHERIOGENOLOGYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectBovineen_US
dc.subjectFrozenen_US
dc.subjectSugarsen_US
dc.subjectGlutamineen_US
dc.subjectFertilityen_US
dc.subjectSpermen_US
dc.titleEffect of glutamine and sugars after bull spermatozoa cryopreservationen_US
dc.typeArticleen_US

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