Testis specific histone 2B is associated with sperm chromatin dynamics and bull fertility-a pilot study

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dc.contributor.authorKutchy, Naseer A.
dc.contributor.authorVelho, Ana
dc.contributor.authorMenezes, Erika S. B.
dc.contributor.authorJacobsen, Marie
dc.contributor.authorThibaudeau, Giselle
dc.contributor.authorWills, Robert W.
dc.contributor.authorMoura, Arlindo
dc.date.accessioned2020-03-26T19:42:46Z
dc.date.available2020-03-26T19:42:46Z
dc.date.issued2017
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractBackground: Bull fertility is the degree of sperm's ability to fertilize and activate the egg and support embryo development, and this is critical for herd reproductive performance. We used the bull as a unique model organism for the study of male fertility because cattle genetics and physiology is similar to those of other mammals including humans. Moreover, reliable fertility data along with well-established in vitro systems are available for bovine. The objective of this original study was to ascertain evolutionary diversification and expression dynamics of Testis Specific Histone 2B (TH2B) in sperm from Holstein bulls with different fertility scores. Methods: The intensity of TH2B was determined by using flow cytometry in sperm from 13 high and 13 low fertility bulls. Expression levels of TH2B were measured using immunofluorescence and Western blotting in sperm from five high and five low fertility bulls. Sequence identity, evolutionary distance and interactome of TH2B were evaluated by dotmatcher, STRING and Cytoscape. Data were analyzed using linear mixed effects model and regression plots were drawn. Results: The intensity of TH2B as measured by flow cytometry was significantly affected by an interaction between fertility group and fertility score (P = 0.0182). The intensity of TH2B in sperm from the high fertility group decreased (P = 0.0055) as fertility increased. TH2B was constantly detectable in sperm and expression levels of TH2B decreased in relation to fertility in sperm from the high fertility group (P = 0.018). TH2B biological functions include male gamete generation, chromosome organization, DNA packaging, DNA conformation change, chromatin organization, nucleosome organization, chromatin disassembly, spermatid nucleus elongation, spermatid nucleus differentiation, sperm motility, chromatin organization, chromatin condensation, chromatin silencing, nucleus organization, and chromatin remodeling (P < 0.05). Conclusions: We elucidated the cellular localization and molecular physiology of TH2B using both computational and cell biology approaches. In addition to advancing the fundamental science of mammalian male gamete, the present findings can be potentially used to evaluate semen quality and predict male fertility in the future.en_US
dc.description.sponsorshipMississippi Agricultural and Forestry Experiment Station, MSU; National Science FoundationNational Science Foundation (NSF) [DBI1262901, EPS 0903787]; Department of Animal and Dairy Sciences, MSU; Brazilian Research Council (CNPq)National Council for Scientific and Technological Development (CNPq); Brazilian Research Council (CAPES)CAPES; Foundation for Research and Scientific Development of the State of Ceara, Brazil (FUNCAP); Netaji Subhas-Indian Council of Agricultural Research for International Fellowships, India (ICAR)en_US
dc.description.sponsorshipMississippi Agricultural and Forestry Experiment Station, MSU (EM). National Science Foundation under award number DBI1262901 (AP). National Science Foundation under award number EPS 0903787 (AP). Department of Animal and Dairy Sciences, MSU (EM). Brazilian Research Councils (CNPq and CAPES) (AM). Foundation for Research and Scientific Development of the State of Ceara, Brazil (FUNCAP) (AM). Netaji Subhas-Indian Council of Agricultural Research for International Fellowships 2015-2017, India (ICAR) (NAK).en_US
dc.identifier.doi10.1186/s12958-017-0274-1en_US
dc.identifier.issn1477-7827en_US
dc.identifier.pmid28764714en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://dx.doi.org/10.1186/s12958-017-0274-1
dc.identifier.urihttps://hdl.handle.net/20.500.12395/35516
dc.identifier.volume15en_US
dc.identifier.wosWOS:000406709300001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherBMCen_US
dc.relation.ispartofREPRODUCTIVE BIOLOGY AND ENDOCRINOLOGYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectHistonesen_US
dc.subjectGene ontologyen_US
dc.subjectMale fertilityen_US
dc.subjectMammalsen_US
dc.subjectSpermen_US
dc.titleTestis specific histone 2B is associated with sperm chromatin dynamics and bull fertility-a pilot studyen_US
dc.typeArticleen_US

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