Immobilization of Candida rugosa lipase on glass beads for enantioselective hydrolysis of racemic Naproxen methyl ester

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dc.contributor.authorYılmaz, Elif
dc.contributor.authorCan, Keziban
dc.contributor.authorSezgin, Mehmet
dc.contributor.authorYılmaz, Mustafa
dc.date.accessioned2020-03-26T18:14:51Z
dc.date.available2020-03-26T18:14:51Z
dc.date.issued2011
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractCandida rugosa lipase (CRL) was immobilized on glutaraldehyde-activated aminopropyl glass beads by using covalent binding method or sal-gel encapsulation procedure and improved considerably by fluoride-catalyzed hydrolysis of mixtures of RSi(OCH3)(3) and Si(OCH3)(4). The catalytic properties of the immobilized lipases were evaluated into model reactions, i.e. the hydrolysis of p-nitrophenylpalmitate (p-NPP). It has been observed that the percent activity yield of the encapsulated lipase was 166.9, which is 5.5 times higher than that of the covalently immobilized lipase. The enantioselective hydrolysis of racemic Naproxen methyl ester by immobilized lipase was studied in aqueous buffer solution/isooctane reaction system and it was noticed that particularly, the glass beads based encapsulated lipases had higher conversion and enantioselectivity compared to covalently immobilized lipase. In short, the study confirms an excellent enantioselectivity (E > 400) for the encapsulated lipase with an ee value of 98% for S-Naproxen. (C) 2010 Elsevier Ltd. All rights reserved.en_US
dc.description.sponsorshipScientific Research Foundation of Selcuk University, Konya, TurkeySelcuk University [08101024]en_US
dc.description.sponsorshipWe thank the Scientific Research Foundation of Selcuk University, Konya, Turkey (BAP-Grant No.: 08101024) for financial support of this work. We also would like to thank Dr. Shahabuddin Memon for her assistance in editing this paper.en_US
dc.identifier.doi10.1016/j.biortech.2010.08.083en_US
dc.identifier.endpage506en_US
dc.identifier.issn0960-8524en_US
dc.identifier.issn1873-2976en_US
dc.identifier.issue2en_US
dc.identifier.pmid20846857en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage499en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.biortech.2010.08.083
dc.identifier.urihttps://hdl.handle.net/20.500.12395/26543
dc.identifier.volume102en_US
dc.identifier.wosWOS:000286782700009en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherELSEVIER SCI LTDen_US
dc.relation.ispartofBIORESOURCE TECHNOLOGYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectCandida rugosa lipaseen_US
dc.subjectGlass beadsen_US
dc.subjectImmobilizationen_US
dc.subjectEnantioselective hydrolysisen_US
dc.subjectS-Naproxenen_US
dc.titleImmobilization of Candida rugosa lipase on glass beads for enantioselective hydrolysis of racemic Naproxen methyl esteren_US
dc.typeArticleen_US

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